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Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm

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Abstract

Objectives

This study sought to investigate, in vitro, the effects of a recently developed triple antibiotic paste (TAP)-mimic polymer nanofibrous scaffold against Porphyromonas gingivalis-infected dentin biofilm.

Materials and methods

Dentin specimens (4 × 4 × 1 mm3) were prepared from human canines. The specimens were sterilized, inoculated with P. gingivalis (ATCC 33277), and incubated for 1 week to allow for biofilm formation. Infected dentin specimens were exposed for 3 days to the following treatments: antibiotic-free polydioxanone scaffold (PDS, control), PDS + 25 wt% TAP [25 mg of each antibiotic (metronidazole, ciprofloxacin, and minocycline) per mL of the PDS polymer solution], or a saturated TAP-based solution (50 mg of each antibiotic per mL of saline solution). In order to serve as the negative control, infected dentin specimens were left untreated (bacteria only). To determine the antimicrobial efficacy of the TAP-mimic scaffold, a colony-forming unit (CFU) per milliliter (n = 10/group) measurement was performed. Furthermore, additional specimens (n = 2/group) were prepared to qualitatively study biofilm inhibition via scanning electron microscopy (SEM). Statistics were performed, and significance was set at the 5 % level.

Results

Both the TAP-mimic scaffold and the positive control (TAP solution) led to complete bacterial elimination, differing statistically (p < 0.05) from the negative control group (bacteria only). No statistical differences were observed for CFU per milliliter data between antibiotic-free scaffolds (2.7 log10 CFU/mL) and the negative control (5.9 log10 CFU/mL).

Conclusions

The obtained data revealed significant antimicrobial properties of the novel PDS-based TAP-mimic scaffold against an established P. gingivalis-infected dentin biofilm.

Clinical relevance

Collectively, the data suggest that the proposed nanofibrous scaffold might be used as an alternative to the advocated clinical gold standard (i.e., TAP) for intracanal disinfection prior to regenerative endodontics.

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References

  1. Cao H, Qi Z, Jiang H, Zhao J, Liu Z, Tang Z (2012) Detection of Porphyromonas endodontalis, Porphyromonas gingivalis and Prevotella intermedia in primary endodontic infections in a Chinese population. Int Endod J 45:773–781. doi:10.1111/j.1365-2591.2012.02035

    Article  PubMed  Google Scholar 

  2. Sassone LM, Fidel R, Faveri M, Fidel S, Figueiredo L, Feres M (2008) Microbiological evaluation of primary endodontic infections in teeth with and without sinus tract. Int Endod J 41:508–515. doi:10.1111/j.1365-2591.2008.01397

    Article  PubMed  Google Scholar 

  3. Rôças IN, Siqueira Jr JF, Santos KR, Coelho AM (2001) “Red complex” (Bacteroides forsythus, Porphyromonas gingivalis, and Treponema denticola) in endodontic infections: a molecular approach. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 91:468–471

    Article  PubMed  Google Scholar 

  4. Qi Z, Cao H, Jiang H, Zhao J, Tang Z (2015) Combinations of bacterial species associated with symptomatic endodontic infections in a Chinese population. Int Endod J. doi:10.1111/iej.12428

    PubMed  Google Scholar 

  5. Stojanović N, Krunić J, Popović B, Stojičić S, Zivković S (2014) Prevalence of Enterococcus faecalis and Porphyromonas gingivalis in infected root canals and their susceptibility to endodontic treatment procedures: a molecular study. Srp Arh Celok Lek 142:535–541

    Article  PubMed  Google Scholar 

  6. Nagata JY, Soares AJ, Souza-Filho FJ, Zaia AA, Ferraz CC, Almeida JF, Gomes BP (2014) Microbial evaluation of traumatized teeth treated with triple antibiotic paste or calcium hydroxide with 2% chlorhexidine gel in pulp revascularization. J Endod 40:778–783. doi:10.1016/j.joen.2014.01.038

    Article  PubMed  Google Scholar 

  7. Martin DE, De Almeida JF, Henry MA, Khaing ZZ, Schmidt CE, Teixeira FB, Diogenes A (2014) Concentration-dependent effect of sodium hypochlorite on stem cells of apical papilla survival and differentiation. J Endod 40:51–55. doi:10.1016/j.joen.2013.07.026

    Article  PubMed  Google Scholar 

  8. Kontakiotis EG, Filippatos CG, Tzanetakis GN, Agrafioti A (2015) Regenerative endodontic therapy: a data analysis of clinical protocols. J Endod 41:146–154

    Article  PubMed  Google Scholar 

  9. Cehreli ZC, Sara S, Aksoy B (2013) Revascularization of immature permanent incisors after severe extrusive luxation injury. J Mich Dent Assoc 95:58–62

    PubMed  Google Scholar 

  10. Saber S-D, El-Hady SA (2012) Development of an intracanal mature Enterococcus faecalis biofilm and its susceptibility to some antimicrobial intracanal medications; an in vitro study. Eur J Dent 6:43–50

    PubMed Central  Google Scholar 

  11. Albuquerque MT, Valera MC, Nakashima M, Nör JE, Bottino MC (2014) Tissue-engineering-based strategies for regenerative endodontics. J Dent Res 93:1222–1231. doi:10.1177/0022034514549809

    Article  PubMed Central  PubMed  Google Scholar 

  12. Diogenes AR, Ruparel NB, Teixeira FB, Hargreaves KM (2014) Translational science in disinfection for regenerative endodontics. J Endod 40:S52–S57. doi:10.1016/j.joen.2014.01.015

    Article  PubMed  Google Scholar 

  13. Hoshino E, Kurihara-Ando N, Sato I, Uematsu H, Sato M, Kota K, Iwaku M (1996) In-vitro antibacterial susceptibility of bacteria taken from infected root dentine to a mixture of ciprofloxacin, metronidazole and minocycline. Int Endod J 29:125–130

    Article  PubMed  Google Scholar 

  14. Althumairy RI, Teixeira FB, Diogenes A (2014) Effect of dentin conditioning with intracanal medicaments on survival of stem cells of apical papilla. J Endod 40:521–525. doi:10.1016/j.joen.2013.11.008

    Article  PubMed  Google Scholar 

  15. Bottino MC, Kamocki K, Yassen GH, Platt JA, Vail MM, Ehrlich Y, Spolnik KJ, Gregory RL (2013) Bioactive nanofibrous scaffolds for regenerative endodontics. J Dent Res 92:963–969. doi:10.1177/0022034513505770

    Article  PubMed  Google Scholar 

  16. Palasuk J, Kamocki K, Hippenmeyer L, Platt JA, Spolnik KJ, Gregory RL, Bottino MC (2014) Bimix antimicrobial scaffolds for regenerative endodontics. J Endod 40:1879–1884. doi:10.1016/j.joen.2014.07.017

    Article  PubMed Central  PubMed  Google Scholar 

  17. Bottino MC, Yassen GH, Platt JA, Labban N, Windsor LJ, Spolnik KJ, Bressiani AH (2013) A novel three-dimensional scaffold for regenerative endodontics: materials and biological characterizations. J Tissue Eng Regen Med. doi:10.1002/term.1712

    PubMed  Google Scholar 

  18. Albuquerque MT, Valera MC, Moreira CS, Bresciani E, de Melo RM, Bottino MC (2015) Effects of ciprofloxacin-containing scaffolds on Enterococcus faecalis biofilms. J Endod 41:710–714. doi:10.1016/j.joen.2014.12.025

    Article  PubMed  Google Scholar 

  19. Albuquerque MTP, Ryan SJ, Münchow EA, Kamocka MM, Gregoy RL, Valera MC, Bottino MC (2015) Antimicrobial effects of novel triple antibiotic paste-mimic scaffolds on Actinomyces naeslundii biofilm. J Endod 24. doi:10.1016/j.joen.2015.03.005

  20. Chandra A (2009) Discuss the factors that affect the outcome of endodontic treatment. Aust Endod J 35:98–107. doi:10.1111/j.1747-4477.2009.00199

    Article  PubMed  Google Scholar 

  21. Saatchi M, Shokraneh A, Navaei H, Maracy MR, Shojaei H (2014) Antibacterial effect of calcium hydroxide combined with chlorhexidine on Enterococcus faecalis: a systematic review and meta-analysis. J Appl Oral Sci 22:356–365

    Article  PubMed Central  PubMed  Google Scholar 

  22. Turk BT, Sen BH, Ozturk T (2009) In vitro antimicrobial activity of calcium hydroxide mixed with different vehicles against Enterococcus faecalis and Candida albicans. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 108:297–301. doi:10.1016/j.tripleo.2009.03.029

    Article  PubMed  Google Scholar 

  23. Ricucci D, Siqueira Jr JF, Lopes WS, Vieira AR, Rôças IN (2015) Extraradicular infection as the cause of persistent symptoms: a case series. J Endod 41:265–273. doi:10.1016/j.joen.2014.08.020

    Article  PubMed  Google Scholar 

  24. Carr GB, Schwartz RS, Schaudinn C, Gorur A, Costerton JW (2009) Ultrastructural examination of failed molar retreatment with secondary apical periodontitis: an examination of endodontic biofilms in an endodontic retreatment failure. J Endod 35:1303–1309. doi:10.1016/j.joen.2009.05.035

    Article  PubMed  Google Scholar 

  25. Cao H, Qi Z, Jiang H, Zhao J, Liu Z, Tang Z (2012) Detection of porphyromonas endodontalis, Porphyromonas gingivalis and Prevotella intermedia in primary endodontic infections in a Chinese population. Int Endod J 45:773–781. doi:10.1111/j.1365-2591.2012.02035

    Article  PubMed  Google Scholar 

  26. Nagata JY, Gomes BP, Rocha Lima TF, Murakami LS, de Faria DE, Campos GR, de Souza-Filho FJ, Soares Ade J (2014) Traumatized immature teeth treated with 2 protocols of pulp revascularization. J Endod 40:606–612. doi:10.1016/j.joen.2014.01.032

    Article  PubMed  Google Scholar 

  27. Damle SG, Bhattal H, Loomba A (2012) Apexification of anterior teeth: a comparative evaluation of mineral trioxide aggregate and calcium hydroxide paste. J Clin Pediatr Dent 36:263–268

    Article  PubMed  Google Scholar 

  28. Torabinejad M, Milan M, Shabahang S, Wright KR, Faras H (2015) Histologic examination of teeth with necrotic pulps and periapical lesions treated with 2 scaffolds: an animal investigation. J Endod 41:846–852. doi:10.1016/j.joen.2015.01.026

    Article  PubMed  Google Scholar 

  29. Chueh LH, Ho YC, Kuo TC, Lai WH, Chen YH, Chiang CP (2009) Regenerative endodontic treatment for necrotic immature permanent teeth. J Endod 35:160–164. doi:10.1016/j.joen.2008.10.019

    Article  PubMed  Google Scholar 

  30. Wang Y, Zhu X, Zhang C (2015) Pulp revascularization on permanent teeth with open apices in a middle-aged patient. J Endod. doi:10.1016/j.joen.2015.04.022

    PubMed Central  Google Scholar 

  31. Sabrah AH, Yassen GH, Gregory RL (2013) Effectiveness of antibiotic medicaments against biofilm formation of Enterococcus faecalis and Porphyromonas gingivalis. J Endod 39:1385–1389. doi:10.1016/j.joen.2013.05.003

    Article  PubMed  Google Scholar 

  32. Akcay M, Arslan H, Yasa B, Kavrık F, Yasa E (2014) Spectrophotometric analysis of crown discoloration induced by various antibiotic pastes used in revascularization. J Endod 40:845–848. doi:10.1016/j.joen.2013.09.019

    Article  PubMed  Google Scholar 

  33. Tanase S, Tsuchiya H, Yao J, Ohmoto S, Takagi N, Yoshida S (1998) Reversed-phase ion-pair chromatographic analysis of tetracycline antibiotics: application to discolored teeth. J Chromatogr B Biomed Sci Appl 706:279–285

    Article  PubMed  Google Scholar 

  34. Ordinola-Zapata R, Bramante CM, Minotti PG, Cavenago BC, Garcia RB, Bernardineli N, Jaramillo DE, Hungaro Duarte MA (2013) Antimicrobial activity of triantibiotic paste, 2% chlorhexidine gel, and calcium hydroxide on an intraoral-infected dentin biofilm model. J Endod 39:115–118. doi:10.1016/j.joen.2012.10.004

    Article  PubMed  Google Scholar 

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Acknowledgments

The authors are grateful to Dr. Eliseu A. Münchow for his help on statistics. This study was funded in part by an International Development Funds (IDF) grant from Indiana University-Purdue University (IUPUI/OVCR) and start-up funds from Indiana University School of Dentistry (IUSD).

Conflict of interests

The authors declare that they have no competing interests.

Compliance with ethical standards

This article does not contain any studies with human participants performed by any of the authors.

Author information

Authors and Affiliations

  1. Department of Biomedical and Applied Sciences, Indiana University School of Dentistry (IUSD), 1121 W. Michigan Street, Indianapolis, IN, 46202, USA

    Maria Tereza P. Albuquerque, Joshua D. Evans, Richard L. Gregory & Marco C. Bottino

  2. Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN, 46202, USA

    Marco C. Bottino

  3. Department of Biomedical Engineering, Purdue School of Engineering and Technology, Indiana University-Purdue University Indianapolis, Indianapolis, IN, 46202, USA

    Marco C. Bottino

  4. Graduate Program in Restorative Dentistry (Endodontics), São José dos Campos Dental School, Universidade Estadual Paulista, São José dos Campos, São Paulo, 12245-000, Brazil

    Maria Tereza P. Albuquerque & Marcia C. Valera

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  1. Maria Tereza P. Albuquerque
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  2. Joshua D. Evans
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  3. Richard L. Gregory
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  4. Marcia C. Valera
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Corresponding author

Correspondence to Marco C. Bottino.

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Albuquerque, M.T.P., Evans, J.D., Gregory, R.L. et al. Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm. Clin Oral Invest 20, 387–393 (2016). https://doi.org/10.1007/s00784-015-1577-2

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  • DOI: https://doi.org/10.1007/s00784-015-1577-2

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