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DNA methylation analysis of SOCS1, SOCS3, and LINE-1 in microdissected gingival tissue

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Abstract

Objectives

DNA methylation plays a critical role in the regulation of the transcription of the suppressors of cytokine signaling (SOCS) 1 and SOCS3, which are modulators in the inflammation. We hypothesized that the methylation status of SOCS1, SOCS3, and long interspersed nuclear element (LINE)-1 in gingival tissues previously inflamed would be similar to that found in gingival tissues without clinical inflammation in the period studied.

Materials and methods

Laser capture microdissection was performed to isolate epithelial and connective gingival tissues. The groups were comprised by ten patients without history of periodontitis and absence of clinical signs of inflammation in the gingiva during the study (healthy group) and ten patients with history of periodontitis, presenting inflammation in the gingival tissue at the first examination of the study (controlled chronic periodontitis group). The gingival biopsies from the controlled chronic periodontitis group were collected after controlling the inflammation. DNA methylation patterns were analyzed using methylation-specific high-resolution melting and combined bisulfite restriction analysis.

Results

DNA methylation levels for SOCS1 and SOCS3 did not differ between groups or tissues; likewise, no differences were observed in total LINE-1 methylation or at specific loci.

Conclusion

At 3 months following control of inflammation in gingival tissues, the methylation profile of SOCS1, SOCS3, and LINE-1 is similar between connective and epithelial tissues from patients that were previously affected or not by chronic inflammation.

Clinical relevance

Clinical results of a successful treatment are observed after inflammation control and the molecular findings illustrate local and general methylation patterns in recovering tissues toward health conditions and might help to understand events that are occurring in oral cells.

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Acknowledgments

This study was supported by a grant from the São Paulo State Research Foundation (FAPESP2010/08180-0). Andia was supported by FAPESP – 2010/02338-0; Planello was supported by FAPESP-2010/05151-9.

Conflict of interest

The authors have no commercial relationships to declare and report no conflicts of interest related to this study. Authors declare do not have a financial relationship with the research foundation that sponsored the research. In addition, they have control of all primary data and they agree to allow the journal to review their data, if requested.

Ethical standard

This study was conducted in accordance with the Helsinki Declaration of 1975, as revised in 2000, and it was independently reviewed and approved by the Ethics Committee for Research of the State University of Campinas (Protocol number 032/2010). Each participant gave their informed consent prior to their inclusion in the study and after explanations were provided.

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Authors and Affiliations

  1. Department of Morphology, Division of Histology, School of Dentistry at Piracicaba, University of Campinas, Av. Limeira 901, 13414-018, Piracicaba, São Paulo-SP, Brazil

    Denise C. Andia, Aline C. Planello, Danielle Portinho, Rodrigo A. da Silva & Ana P. de Souza

  2. Department of Prosthodontics and Periodontics, Division of Periodontics, School of Dentistry at Piracicaba, University of Campinas, Piracicaba, São Paulo-SP, Brazil

    Cristiane R. Salmon, Enilson A. Sallum & Francisco H. Nociti Junior

Authors
  1. Denise C. Andia
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  2. Aline C. Planello
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  4. Rodrigo A. da Silva
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  7. Francisco H. Nociti Junior
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  8. Ana P. de Souza
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Correspondence to Denise C. Andia.

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Andia, D.C., Planello, A.C., Portinho, D. et al. DNA methylation analysis of SOCS1, SOCS3, and LINE-1 in microdissected gingival tissue. Clin Oral Invest 19, 2337–2344 (2015). https://doi.org/10.1007/s00784-015-1460-1

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  • DOI: https://doi.org/10.1007/s00784-015-1460-1

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