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Synchrotron-based X-ray tomographic microscopy for visualization of three-dimensional collagen matrices

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Clinical Oral Investigations Aims and scope Submit manuscript

Abstract

Introduction

Three-dimensional collagen matrices (3D-CMs) may be visualized by cumbersome reconstructions of serial sections. We report here on the method of synchrotron-based X-ray tomographic microscopy (SRXTM) to image 3D-CMs in native tissue probes.

Material and methods

SRXTM of 3D-CMs (mucoderm®, mucograft®) was performed at the TOMCAT beamline of the Swiss Light Source (SLS) at the Paul Scherrer Institute (Villigen, Switzerland).

Results

SRXTM combines the advantages of high-resolution scanning electron microscopy (SEM) imaging with the low-resolution reconstructions of micro-CT (μCT) imaging. It may be used to non-destructively visualize and analyze structures within the 3D-CMs without the need of serial sectioning and reconstruction.

Conclusion

High-resolution SRXTM is a useful tool in analyzing the topology and morphometry of structures in 3D-CMs. The outcome justifies the efforts in sophisticated data processing.

Clinical relevance

SRXTM may help to understand the clinical characteristics of 3D-CMs in more detail.

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References

  1. Pabst AM, Happe A, Callaway A, Ziebart T, Stratul SI, Ackermann M, Konerding MA, Willershausen B, Kasaj A (2014) In vitro and in vivo characterization of porcine acellular dermal matrix for gingival augmentation procedures. J Periodontal Res 49:371–381

    Article  PubMed  Google Scholar 

  2. Ghanaati S, Schlee M, Webber MJ, Willershausen I, Barbeck M, Balic E, Görlach C, Stupp SI, Sader RA, Kirkpatrick CJ (2011) Evaluation of the tissue reaction to a new bilayered collagen matrix in vivo and its translation to the clinic. Biomed Mater 6:015010

    Article  PubMed  Google Scholar 

  3. Stampanoni M, Groso A, Isenegger A, Mikuljan G, Chen Q, Bertrand A, Henein S, Betemps R, Frommherz U, Böhler P, Meister D, Lange M, Abela R (2006) Trends in synchrotron-based tomographic imaging: the SLS experience. Proc. SPIE 6318, Developments in X-Ray Tomography V, 63180M

  4. Hintermüller C, Marone F, Isenegger A, Stampanoni M (2010) Image processing pipeline for synchrotron-radiation-based tomographic microscopy. J Synchrotron Radiat 17:550–559

    Article  PubMed  Google Scholar 

  5. Marone F, Stampanoni M (2012) Regridding reconstruction algorithm for real-time tomographic imaging. J Synchrotron Radiat 19:1029–1037

    Article  PubMed Central  PubMed  Google Scholar 

  6. Rack A, Weitkamp T, Riotte M, Grigoriev D, Rack T, Helfen L, Baumbach T, Dietschn R, Holz T, Kramer M (2010) Comparative study of multilayers used in monochromators for synchrotron-based coherent hard x-ray imaging. J Synchrotron Radiat 17:496–510

    Article  PubMed  Google Scholar 

  7. Haberthür D, Hintermüller C, Marone F, Schittny JC, Stampanoni M (2010) Radiation dose optimized lateral expansion of the field of view in synchrotron radiation X-ray tomographic microscopy. J Synchrotron Radiat 17:590–599

    Article  PubMed Central  PubMed  Google Scholar 

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Acknowledgments

Synchrotron-based X-ray tomographic microscopy (SRXTM) experiments were facilitated by a research grant of the Swiss Light Source at the Paul Scherrer Institute (Villigen, Switzerland) to A.M. Pabst. The research leading to these results has received funding from the European Community’s Seventh Framework Programme (FP7/2007-2013) under grant agreement no. 312284 for CALIPSO.

Conflict of interests

The authors declare no conflict of interests.

Author information

Authors and Affiliations

  1. Institute of Functional and Clinical Anatomy, University Medical Center Mainz, Becherweg 13, 55099, Mainz, Germany

    A. M. Pabst, W. Wagner, M. Ackermann & Moritz A. Konerding

  2. Department of Oral and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, 55131, Mainz, Germany

    A. M. Pabst

  3. Swiss Light Source, Paul Scherrer Institute (PSI), 5232, Villigen, Switzerland

    A. Astolfo, F. Marone & D. Haberthür

  4. Institute of Geosciences, Johannes Gutenberg University of Mainz, Johann-Joachim-Becher-Weg 21, 55099, Mainz, Germany

    F. Enzmann

  5. Department of Operative Dentistry, University Medical Center Mainz, Augustusplatz 2, 55131, Mainz, Germany

    A. Kasaj

  6. Institute of Sport Sciences, Division for Rehabilitation and Sports Medicine, Johannes Gutenberg University of Mainz, Albert-Schweitzer-Str. 22, 55128, Mainz, Germany

    S. Gebhardt

Authors
  1. A. M. Pabst
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  2. W. Wagner
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  3. A. Kasaj
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  4. S. Gebhardt
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  5. M. Ackermann
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  6. A. Astolfo
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  7. F. Marone
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  8. D. Haberthür
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  9. F. Enzmann
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  10. Moritz A. Konerding
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Corresponding author

Correspondence to Moritz A. Konerding.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Movie 1

Overview of a mucoderm® scan (white bar = 1,250 μm) (MPG 123,946 kb)

Movie 2

View on the surface layer of a mucoderm® scan in higher magnification (white bar = 400 μm) (MPG 244,052 kb)

Movie 3

Overview of a mucograft® scan (spongious layer; white bar = 1,200 μm) (MPG 114,236 kb)

Movie 4

Overview of the surface layer of a mucograft® scan (compact layer; white bar = 350 μm) (MPG 163,838 kb)

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Cite this article

Pabst, A.M., Wagner, W., Kasaj, A. et al. Synchrotron-based X-ray tomographic microscopy for visualization of three-dimensional collagen matrices. Clin Oral Invest 19, 561–564 (2015). https://doi.org/10.1007/s00784-014-1312-4

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  • DOI: https://doi.org/10.1007/s00784-014-1312-4

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