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In vitro evaluation of anticancer and antibacterial activities of cobalt oxide nanoparticles

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Abstract

Cobalt oxide nanoparticles (Co3O4-NPs) were synthesized using simple urea-based thermal decomposition method. Phase purity and particle size of as-synthesized nanoparticles were characterized through X-ray diffraction pattern (XRD) and transmission electron microscopy. Through XRD morphology of the Co3O4-NPs was found to be variable in size with range of 36 nm. In our present study, we explored the potential cytotoxic and antibacterial effects of Co3O4-NPs in human colorectal types of cancerous cells (HT29 and SW620) and also nine Gram-positive and Gram-negative bacteria. Co3O4-NPs showed promising anticancer activity against HT29 and SW620 cells with IC50 value of 2.26 and 394.5 μg/mL, respectively. However, no significant effect of Co3O4-NPs was observed against bacterial strains. Furthermore, a detailed study has been carried out to investigate the possible mechanism of cell death in HT29 cancer cell line through the analysis of expression level of anti-apoptotic Bcl2 and BclxL markers. Western blot analysis results suggested significant role of Co3O4-NPs exposure in cell death due to apoptosis.

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Acknowledgments

The authors express gratefulness to the Research Center, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.

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Authors and Affiliations

  1. Nanomedicine and Biotechnology Research Unit, Department of Pharmaceutics, College of Pharmacy, King Saud University, PO Box 2457, Riyadh, 11451, Saudi Arabia

    Shahanavaj Khan & Abdul Arif Khan

  2. Department of Bioscience, Shri Ram Group of College (SRGC), Muzaffarnagar, UP, India

    Shahanavaj Khan

  3. King Abdullah Institute for Nanotechnology, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia

    Anees A. Ansari

  4. Colorectal Research Center, College of Medicine, King Saud University, Riyadh, 11451, Saudi Arabia

    Rehan Ahmad & Omar Al-Obaid

  5. Department of Surgery, Al-Faisal University Riyadh, Riyadh, 11451, Saudi Arabia

    Wael Al-Kattan

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  1. Shahanavaj Khan
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  3. Abdul Arif Khan
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Correspondence to Shahanavaj Khan.

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Electronic supplementary material

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Supplementary material 1 (DOC 30 kb) Fig. S1 XRD pattern of the cobalt oxide nanoparticles (Co3O4-NPs)

775_2015_1310_MOESM2_ESM.doc

Supplementary material 2 (DOC 2767 kb) Fig. S2 Synthesized Co3O4 napoparticles illustrate negligible antibacterial potential against four Gram-positive (Escherichia coli ATCC-35218, Escherichia coli ATCC-25922, Enterococcus faecalis ATCC-29212, and Bacillus subtilis NCTC-10400 and five Gram-negative bacteria (Staphylococcus aureus ATCC-29213, Pseudomonas aeruginosa ATCC-27853, Shigella sonnei ATCC-11060, Salmonella typhimurium ATCC-13311, and Proteus vulgaris ATCC-6380)) in 250 μg/mL (as indicated by T) concentration with comparison to control drugs Ampicillin: AM and Cefotaxime: CTX (as indicated by C)

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Khan, S., Ansari, A.A., Khan, A.A. et al. In vitro evaluation of anticancer and antibacterial activities of cobalt oxide nanoparticles. J Biol Inorg Chem 20, 1319–1326 (2015). https://doi.org/10.1007/s00775-015-1310-2

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  • DOI: https://doi.org/10.1007/s00775-015-1310-2

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