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Alkaline phosphatase determines polyphosphate-induced mineralization in a cell-type independent manner

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Abstract

Polyphosphate [Poly(P)] has positive effects on osteoblast mineralization; however, the underlying mechanism remains unclear. In addition, it is unknown whether Poly(P) promotes mineralization in soft tissues. We investigated this by using various cells. Poly(P) concentrations of 1 and 0.5 mg/mL yielded high levels of mineralization in ROS17/2.8 osteoblast cells. Similarly, Poly(P) induced mineralization in cell types expressing alkaline phosphatase (ALP), namely, ATDC5 and MC3T3-E1, but not in CHO, C3H10T1/2, C2C12, and 3T3-L1 cells. Furthermore, forced expression of ALP caused Poly(P)-induced mineralization in CHO cells. These results suggest that ALP determines Poly(P)-induced mineralization in a cell-type independent manner.

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Acknowledgments

This work was supported in part by the Sato Fund, Nihon University School of Dentistry (2010), a Strategic Research Base Development Program for Private Universities from the Ministry of Education, Culture, Sports, Science and Technology of Japan, 2010-2014 (S1001024) and a Nihon University Joint Research Grant (2012).

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Authors and Affiliations

  1. Department of Pathology, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan

    Yoshikazu Mikami & Kazuo Komiyama

  2. Department of Biochemistry, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan

    Hiromasa Tsuda & Naoto Suzuki

  3. Department of Orthodontics, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan

    Yuko Akiyama & Noriyoshi Shimizu

  4. Department of Oral Anatomy, Aichi-Gakuin University School of Dentistry, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi, 464-8650, Japan

    Masaki Honda

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  1. Yoshikazu Mikami
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  2. Hiromasa Tsuda
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Correspondence to Hiromasa Tsuda.

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Supplemental Fig. 1. Effects of Poly(P) on the expression of osteogenic genes in CHO, C3H10T1/2, C2C12, 3T3-L1, ATDC5, and MC3T3-E1 cells. Cells were cultured with or without Poly(P) (1 mg/mL) in mineralization medium for 14 days. Thereafter, mRNA expression of osteogenic transcription factors (Runx2 and osterix) and osteoblast markers (bone sialoprotein (BSP), osteocalcin (OCN), and collagen type I (Col.I)) was analyzed by RT-PCR.

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Mikami, Y., Tsuda, H., Akiyama, Y. et al. Alkaline phosphatase determines polyphosphate-induced mineralization in a cell-type independent manner. J Bone Miner Metab 34, 627–637 (2016). https://doi.org/10.1007/s00774-015-0719-6

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  • DOI: https://doi.org/10.1007/s00774-015-0719-6

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