Abstract
Pyrococcus horikoshii lysyl-tRNA synthetase/tRNA orthogonal pair exhibited high selectivity towards d-lysine in the presence of excess amount of d-lysine. Based on the observation, this orthogonal pair was employed to encode d-lysine, and d-lysine was site-specifically incorporated into the diketoreductase in E. coli cells.
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Acknowledgments
We are grateful for the technical assistance from Zhijuan Xu and Hairong Ma. This work was supported by “111 project” from the Ministry of Education of China and the State Administration of Foreign Export Affairs of China (No: 111-2-07) and the Innovation Fund Project for Graduate Students of Jiangsu Province (CXLX11-0796).
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Liu, Z., Yang, X., Yi, D. et al. Genetic incorporation of d-lysine into diketoreductase in Escherichia coli cells. Amino Acids 43, 2553–2559 (2012). https://doi.org/10.1007/s00726-012-1311-1
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DOI: https://doi.org/10.1007/s00726-012-1311-1