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Peroxisomal NADP-isocitrate dehydrogenase is required for Arabidopsis stomatal movement

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Abstract

Peroxisomes are subcellular organelles characterized by a simple morphological structure but have a complex biochemical machinery involved in signaling processes through molecules such as hydrogen peroxide (H2O2) and nitric oxide (NO). Nicotinamide adenine dinucleotide phosphate (NADPH) is an essential component in cell redox homeostasis, and its regeneration is critical for reductive biosynthesis and detoxification pathways. Plants have several NADPH-generating dehydrogenases, with NADP-isocitrate dehydrogenase (NADP-ICDH) being one of these enzymes. Arabidopsis contains three genes that encode for cytosolic, mitochondrial/chloroplastic, and peroxisomal NADP-ICDH isozymes although the specific function of each of these remains largely unknown. Using two T-DNA insertion lines of the peroxisomal NADP-ICDH designated as picdh-1 and picdh-2, the data show that the peroxisomal NADP-ICDH is involved in stomatal movements, suggesting that peroxisomes are a new element in the signaling network of guard cells.

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Acknowledgments

ML acknowledges a JAE-Doc contract from CSIC. Microscopy analyses were carried out at the Technical Services of the University of Granada and Estación Experimental del Zaidín (CSIC). Special thanks are given to Mr. Carmelo Ruíz-Torres for his excellent technical support. T-DNA insertion mutant seeds were provided by the Nottingham Arabidopsis Stock Centre, UK. This work was supported by ERDF-cofinanced grants from the Ministry of Economy and Competitiveness (BIO2012-33904) and Junta de Andalucía (groups BIO192 and BIO 286).

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The authors declare that they have no conflict of interests.

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Authors and Affiliations

  1. Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Apartado 419, 18080, Granada, Spain

    Marina Leterrier, Mounira Chaki, José M. Palma & Francisco J. Corpas

  2. Group of Biochemistry and Cell Signaling in Nitric Oxide, Department of Biochemistry and Molecular Biology, University of Jaén, Campus “Las Lagunillas”, 23071, Jaén, Spain

    Juan B. Barroso, Raquel Valderrama, Juan C. Begara-Morales, Beatriz Sánchez-Calvo & Francisco Luque

  3. Departamento de Biología Animal, Biología Vegetal y Ecología (Ecología), Facultad de Ciencias Experimentales, Universidad de Jaén, Jaén, Spain

    Benjamin Viñegla

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  1. Marina Leterrier
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  2. Juan B. Barroso
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  3. Raquel Valderrama
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  4. Juan C. Begara-Morales
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  5. Beatriz Sánchez-Calvo
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  6. Mounira Chaki
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  7. Francisco Luque
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  8. Benjamin Viñegla
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  9. José M. Palma
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  10. Francisco J. Corpas
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Corresponding author

Correspondence to Francisco J. Corpas.

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Leterrier, M., Barroso, J.B., Valderrama, R. et al. Peroxisomal NADP-isocitrate dehydrogenase is required for Arabidopsis stomatal movement. Protoplasma 253, 403–415 (2016). https://doi.org/10.1007/s00709-015-0819-0

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