Abstract
The morphology and diffusional water permeability (P d) of red blood cells (RBCs) from green sea turtle (GST) (Chelonia mydas) are presented for the first time. The RBCs had an ellipsoidal shape with full-axis lengths (diameters): D = 14.4 μm; d = 10.2 μm; h = 2.8 μm. The values of P d (cm s−1) were 5.1 × 10−3 at 15 °C, 5.7 × 10−3 at 20 °C, 6.3 × 10−3 at 25 °C, 6.8 × 10−3 at 30 °C, and 7.9 × 10−3 at 37 °C (i.e., significantly higher than in human RBCs in which it was measured to be 4.2 × 10−3 at 25 °C, 5.0 × 10−3 at 30 °C, and 6.2 × 10−3 at 37 °C). There was a lack of inhibition of P d of GST RBCs by p-chloromercuribenzoate (PCMB), a well-known inhibitor of the RBC water channel proteins (WCPs). The activation energy of water diffusion (E a,d) in GST RBCs was 15.0 ± 1.6 kJ mol−1 which is lower than the E a,d for human RBCs (~25 kJ mol−1). These results indicate that in the membrane of GST RBCs, there were no WCPs that were inhibited by the mercurial reagent, while the lipid bilayer of this membrane is unusually permeable to water. This is likely to be a phylogenetically old trait, like that found in amphibians and even the later birds, all of which have nucleated erythrocytes; and it is also likely to be a result of the animal’s adaptation to a herbivorous diet (algae and seagrasses).
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Acknowledgments
We thank Dr William Bubb and Mr William Lowe (University of Sydney), Dr Gheorghe Zsolt Nicula, Mrs Adina Chiş and Mr Radu Munteanu (Discipline of Cell and Molecular Biology, “Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania), for assistance with experimental, computer, and secretarial work. We record our appreciation to the late Professor C. H. Gallagher who through Taronga Zoo, Sydney, arranged for the blood samples and to the late Dr Bogdan E. Chapman for his contribution to the NMR analyses.
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Benga, G., Chapman, B.E., Romeo, T. et al. Morphology and water permeability of red blood cells from green sea turtle (Chelonia mydas). Protoplasma 252, 1181–1185 (2015). https://doi.org/10.1007/s00709-014-0747-4
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DOI: https://doi.org/10.1007/s00709-014-0747-4