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Expression and functional analysis of putative vacuolar Ca2+-transporters (CAXs and ACAs) in roots of salt tolerant and sensitive rice cultivars

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Abstract

Vacuolar Ca2+-transporters could play an important role for salt tolerance in rice (Oryza sativa L.) root. Here, we compared the expression profiles of putative vacuolar cation/H+ exchanger (CAX) and calmodulin-regulated autoinhibited Ca2+-ATPase (ACA) in rice roots of salt tolerant cv. Pokkali and salt sensitive cv. IR29. In addition to five putative vacuolar CAX genes in the rice genome, a new CAX gene (OsCAX4) has been annotated. In the present study, we isolated the OsCAX4 gene and showed that its encoded protein possesses a unique transmembrane structure and is potentially involved in transporting not only Ca2+ but also Mn2+ and Cu2+. These six OsCAX genes differed in their mRNA expression pattern in roots of tolerant versus sensitive rice cultivars exposed to salt stress. For example, OsCAX4 showed abundant expression in IR29 (sensitive) upon prolonged salt stress. The mRNA expression profile of four putative vacuolar Ca2+-ATPases (OsACA4-7) was also examined. Under control conditions, the mRNA levels of OsACA4, OsACA5, and OsACA7 were relatively high and similar among IR29 and Pokkali. Upon salt stress, only OsACA4 showed first a decrease in its expression in Pokkali (tolerant), followed by a significant increase. Based on these results, a role of vacuolar Ca2+ transporter for salt tolerance in rice root was discussed.

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Abbreviations

ACAs:

autoinhibited calcium ATPase

CAXs:

Ca2+/H+ exchangers

TM:

transmembrane

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Acknowledgments

This work was supported by postdoctoral program of the National Science and Technology Development Agency (NSTDA), Thailand to NY and partially granted by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan and the International Center for Green Biotechnology of Meijo University. The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Plant Physiology and Biochemistry Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand

    Nana Yamada, Cattarin Theerawitaya, Suriyan Cha-um & Chalermpol Kirdmanee

  2. Research Institute, Meijo University, Tenpaku-ku, Nagoya, Aichi, 468-8502, Japan

    Teruhiro Takabe

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  1. Nana Yamada
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  2. Cattarin Theerawitaya
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  3. Suriyan Cha-um
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  4. Chalermpol Kirdmanee
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  5. Teruhiro Takabe
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Correspondence to Teruhiro Takabe.

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Yamada, N., Theerawitaya, C., Cha-um, S. et al. Expression and functional analysis of putative vacuolar Ca2+-transporters (CAXs and ACAs) in roots of salt tolerant and sensitive rice cultivars. Protoplasma 251, 1067–1075 (2014). https://doi.org/10.1007/s00709-014-0615-2

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