Skip to main content
SpringerLink
Log in

Native and denatured enzyme enterokinase determined by electrochemical methods

  • Original Paper
  • Published:
Monatshefte für Chemie - Chemical Monthly Aims and scope Submit manuscript

Abstract

The aim of this work is to detect and distinguish native and denatured form of enzyme enteropeptidase. Cyclic voltammetry is usually the method of choice to be used in experiments with biomolecules or unknown chemical species. However, only hardly detectable differences between native and denatured form of enzyme enterokinase were observed using cyclic voltammetry. Therefore, chronopotentiometric stripping analysis was used for the characterization of protein conformation. Significant differences between the heights of peak H of native and denatured form of enterokinase were observed. Our experiments have proved that constant current chronopotentiometric peak H at mercury electrode is sensitive tool for the characterization of changes in protein conformation.

Graphical abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Pospisil L, Hromadova M, Gal M, Bulickova J, Sokolova R, Fanelli N (2008) Electrochim Acta 53:7445

    Article  CAS  Google Scholar 

  2. Pospisil L, Hromadova M, Fanelli N, Valasek M, Kolivoska V, Gal M (2011) Phys Chem Chem Phys 13:4365

    Article  CAS  Google Scholar 

  3. Hromadova M, Kolivoska V, Sokolova R, Gal M, Pospisil L, Valasek M (2010) Langmuir 26:17232

    Article  CAS  Google Scholar 

  4. Pospisil L, Gal M, Hromadova M, Bulickova J, Kolivoska V, Cvacka J, Novakova K, Kavan L, Zukalova M, Dunsch L (2010) Phys Chem Chem Phys 12:14095

    Article  CAS  Google Scholar 

  5. Pospisil L, Hromadova M, Gal M, Valasek M, Fanelli N, Kolivoska V (2009) Collect Czech Chem Commun 74:1559

    Article  CAS  Google Scholar 

  6. Gal M, Kielar F, Sokolova R, Ramesova S, Kolivoska V (2013) Eur J Inorg Chem 18:3217

    Article  Google Scholar 

  7. Kolivoska V, Gal M, Hromadova M, Lachmanova S, Tarabkova H, Janda P, Pospisil L, Turonova AM (2012) Colloids Surf B-Biointerfaces 94:213

    Article  CAS  Google Scholar 

  8. Barek J, Fischer J, Navratil T, Peckova K, Yosypchuk B, Zima J (2007) Electroanalysis 19:2003

    Article  CAS  Google Scholar 

  9. Fadrna R, Yosypchuk B, Fojta M, Navratil T, Novotny L (2004) Anal Lett 37:399

    Article  CAS  Google Scholar 

  10. Navratil T, Yosypchuk B, Barek J (2009) Chem Anal (Warsaw) 54:3

    CAS  Google Scholar 

  11. Selesovska R, Bandzuchova L, Navratil T, Chylkova J (2012) Electrochim Acta 60:375

    Article  CAS  Google Scholar 

  12. Skopalova J, Navratil T (2007) Chem Anal (Warsaw) 52:961

    CAS  Google Scholar 

  13. Palecek E, Ostatna V (2007) Electroanalysis 19:2383

    Article  CAS  Google Scholar 

  14. Palecek E (1996) Electroanalysis 8:7

    Article  CAS  Google Scholar 

  15. Palecek E, Fojta M (2001) Anal Chem 73:74a

    CAS  Google Scholar 

  16. Ostatna V, Kuralay F, Trnkova L, Palecek E (2008) Electroanalysis 20:1406

    Article  CAS  Google Scholar 

  17. Ostatna V, Palecek E (2008) Electrochim Acta 53:4014

    Article  CAS  Google Scholar 

  18. Honeychurch MJ (1997) Bioelectrochem Bioenerg 44:13

    Article  CAS  Google Scholar 

  19. Brdička R (1933) Collect Czech Chem Commun 5:148

    Article  Google Scholar 

  20. Ostatna V, Uslu B, Dogan B, Ozkan S, Palecek E (2006) J Electroanal Chem 593:172

    Article  CAS  Google Scholar 

  21. Palecek E, Ostatna V, Masarik M, Bertoncini CW, Jovin TM (2008) Analyst 133:76

    Article  CAS  Google Scholar 

  22. Tomschik M, Havran L, Fojta M, Palecek E (1998) Electroanalysis 10:403

    Article  CAS  Google Scholar 

  23. Kizek R, Trnkova L, Palecek E (2001) Anal Chem 73:4801

    Article  CAS  Google Scholar 

  24. Tomschik M, Havran L, Palecek E, Heyrovsky M (2000) Electroanalysis 12:274

    Article  CAS  Google Scholar 

  25. Jirickova K, Gal M, Krahulec J, Hives J (2015) Monatsh Chem 146:755

    Article  CAS  Google Scholar 

  26. Jirickova K, Krahulec J, Levarski Z, Hopkova D, Stuchlik S, Turna J (2014) FEBS J 281:667

    Google Scholar 

  27. Pepeliaev S, Krahulec J, Cerny Z, Jilkova J, Tlusta M, Dostalova J (2011) J Biotechnol 156:67

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research was supported by the Slovak Research and Development Agency under the contract No. APVV-0119-12. This publication is also the result of the project implementation: Centre for materials, layers and systems for applications and chemical processes under extreme conditions-Stage II, ITMS No. 26240120021 supported by the Research and Development Operational Programme funded by the ERDF.

Author information

Authors and Affiliations

  1. Faculty of Chemical and Food Technology, Institute of Inorganic Chemistry, Technology and Materials, Slovak University of Technology in Bratislava, Radlinského 9, 812 37, Bratislava, Slovakia

    Alica Janovjáková, Miroslav Gál & Ján Híveš

  2. Department of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovičova 6, 842 15, Bratislava 4, Slovakia

    Ján Krahulec

  3. J. Heyrovský Institute of Physical Chemistry CAS, v.v.i., Dolejškova 3, 182 23, Prague, Czech Republic

    Romana Sokolová

  4. Institute of Chemistry, University in Bialystok, K.Ciolkowskiego 1 K, 15-245, Białystok, Poland

    Monika Naumowicz

Authors
  1. Alica Janovjáková
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Miroslav Gál
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ján Krahulec
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Romana Sokolová
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Monika Naumowicz
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ján Híveš
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Miroslav Gál.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Janovjáková, A., Gál, M., Krahulec, J. et al. Native and denatured enzyme enterokinase determined by electrochemical methods. Monatsh Chem 148, 549–553 (2017). https://doi.org/10.1007/s00706-016-1915-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00706-016-1915-3

Keywords

Search

Navigation