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Detection of koi herpesvirus (KHV) using a monoclonal antibody against Cyprinus carpio IgM

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Abstract

Koi herpesvirus disease (KHVD) is associated with high mortality in both common carp and koi carp (Cyprinus carpio L.) worldwide. The indirect detection of fish viruses based on the identification of antibodies has emerged as a practical and reliable means of diagnosis. Thus, it is important to create monoclonal antibodies (MAbs) against carp IgM. By using hybridoma-monoclonal antibody technology, one hybridoma cell line secreting MAbs against IgM from carp was established. In western blot analysis, the secreted MAb from cell line A5-E10 recognized the heavy chain of IgM from common carp or koi but did not react with immunoglobulins from three different fish species: grass carp (Ctenopharyngodon idella), tilapia (Oreochromis mossambicus) and Mandarin fish (Siniperca chuatsi). These results demonstrated that this MAb is highly specific for the IgM of carp and suggested that it can be used for monitoring the immunity level of carp, for example for indirect KHV diagnosis by antibody ELISA. We therefore established an indirect ELISA, which was tested using 200 serum samples from koi from three farms. The final results showed that 147 (73.5%) samples were confirmed to be KHV antibody negative and 53 (26.5%) were definitely positive, containing antibodies against KHV.

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Authors and Affiliations

  1. Key Laboratory of Fishery Drug Development of Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Liwan District, Guangzhou, 510380, Guangdong, The People’s Republic of China

    Yingying Li, Shucheng Zheng, Qing Wang, Weiwei Zeng, Yingying Wang, Chun Liu & Cunbin Shi

  2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, The People’s Republic of China

    Shucheng Zheng

  3. German Reference Laboratory for KHVD, Institute of Infectology, Friedrich-Loeffler-Institut (FLI), Federal Research Institute for Animal Health, 17493, Greifswald-Insel Riems, Germany

    Sven M. Bergmann

Authors
  1. Yingying Li
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  2. Shucheng Zheng
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  3. Qing Wang
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  4. Sven M. Bergmann
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  5. Weiwei Zeng
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  6. Yingying Wang
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  7. Chun Liu
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  8. Cunbin Shi
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Corresponding author

Correspondence to Qing Wang.

Ethics declarations

The use of the mice and fish in this study was approved by the aquatic animal research committee at Pearl River Fisheries Research Institute. All experimental procedures were conducted in conformity with institutional guidelines for care and use of laboratory animals in Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, China. All experiments were also performed according to the Chinese Association for Laboratory Animal Sciences guidelines for care and use of laboratory animals (IACUC).

Funding

This study was funded by the Central Public-Interest Scientific Institution Basal Research Fund, CAFS (No. 2017GH01), Science and Industry Development from the Administration of Ocean and Fisheries of Guangdong Province in China (No. A201501B13) and China Agriculture Research System (No. CARS-46).

Conflict of interest

We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work; there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the position presented in, or the review of, the manuscript.

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Li, Y., Zheng, S., Wang, Q. et al. Detection of koi herpesvirus (KHV) using a monoclonal antibody against Cyprinus carpio IgM. Arch Virol 162, 2381–2385 (2017). https://doi.org/10.1007/s00705-017-3357-6

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  • DOI: https://doi.org/10.1007/s00705-017-3357-6

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