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Molecular characterization of a new soybean-infecting member of the genus Nepovirus identified by high-throughput sequencing

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Abstract

The complete nucleotide sequence of a new soybean-infecting member of the genus Nepovirus (provisionally named “soybean latent spherical virus” [SLSV]) was identified by high-throughput sequencing of RNAs from soybean leaf samples from North Dakota, USA. The sequences of RNAs 1 (8,190 nt) and 2 (5,788 nt) were completed by rapid amplification of cDNA ends. Each contained a single long open reading frame and a 3’ nontranslated region of greater than 1,500 nt. The predicted amino acid sequences of the two ORFs were most closely related to nepoviruses in subgroup C. Full-length cDNAs of RNAs 1 and 2 were cloned and used to inoculate soybean plants, which did not display obvious symptoms. These results suggest that SLSV represents a new species in the genus Nepovirus.

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References

  1. Sanfaçon H, Iwanami T, Karasev AV, van der Vlugt R, Wellink J, Wetzel T, Yoshikawa N (2012) Family Secoviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy: classification and nomenclature of viruses; ninth report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, Waltham, pp 1139–1162

    Google Scholar 

  2. Messieha M (1969) Transmission of tobacco ringspot virus by Thrips. Phytopathology 59:943–945

    CAS  PubMed  Google Scholar 

  3. Susi P (2004) Black currant reversion virus, a mite-transmitted Nepovirus. Mol Plant Pathol 5:167–173

    Article  CAS  PubMed  Google Scholar 

  4. Martelli GP (1978) Nematode-borne viruses of grapevine, their epidemiology and control. Nematol Mediterr 6:1–27

    Google Scholar 

  5. Isogai M, Tatuto N, Ujiie C, Watanabe M, Yoshikawa N (2012) Identification and characterization of blueberry latent spherical virus, a new member of subgroup C in the genus Nepovirus. Arch Virol 157:297–303

    Article  CAS  PubMed  Google Scholar 

  6. Hartman GL, Rupe JC, Sikora EF, Domier LL, Davis JA, Steffey KL (2015) Compendium of soybean diseases and pests. APS Press, American Phytopathological Society, St. Paul

  7. Marzano SL, Nelson BD, Ajayi-Oyetunde O, Bradley CA, Hughes TJ, Hartman GL, Eastburn DE, Domier LL (2016) Identification of diverse mycoviruses through metatranscriptomics characterization of the viromes of five major fungal plant pathogens. J Virol (in press)

  8. Scholthof HB (1999) Rapid delivery of foreign genes into plants by direct rub-inoculation with intact plasmid DNA of a tomato bushy stunt virus gene vector. J. Virol 73:7823–7829

    CAS  PubMed  PubMed Central  Google Scholar 

  9. Domier LL, Hobbs HA, McCoppin NK, Bowen CR, Steinlage TA, Chang S, Wang Y, Hartman GL (2011) Multiple loci condition seed transmission of Soybean mosaic virus (SMV) and SMV-induced seed coat mottling in soybean. Phytopathology 101:750–756

    Article  CAS  PubMed  Google Scholar 

  10. Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Childress AM, Ramsdell DC (1987) Bee-mediated transmission of blueberry leaf mottle virus via infected pollen in highbush blueberry. Phytopathology 77:167–172

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Crop Sciences, University of Illinois, Urbana, IL, USA

    Tuba Yasmin, Houston A. Hobbs, Kris N. Lambert & Leslie L. Domier

  2. Department of Plant Pathology, North Dakota State University, Fargo, ND, USA

    Berlin D. Nelson

  3. United States Department of Agriculture, Agricultural Research Service, Urbana, IL, USA

    Nancy K. McCoppin & Leslie L. Domier

Authors
  1. Tuba Yasmin
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  2. Berlin D. Nelson
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  3. Houston A. Hobbs
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  4. Nancy K. McCoppin
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  5. Kris N. Lambert
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  6. Leslie L. Domier
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Corresponding author

Correspondence to Leslie L. Domier.

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Funding

This study was funded by the United States Department of Agriculture/Agricultural Research Service and the North Dakota Soybean Growers Association. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the United States Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that may also be suitable.

Conflict of interest

The authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Yasmin, T., Nelson, B.D., Hobbs, H.A. et al. Molecular characterization of a new soybean-infecting member of the genus Nepovirus identified by high-throughput sequencing. Arch Virol 162, 1089–1092 (2017). https://doi.org/10.1007/s00705-016-3152-9

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  • DOI: https://doi.org/10.1007/s00705-016-3152-9

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