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Isolation and characterization of a new enterovirus F in yak feces in the Qinghai-Tibetan Plateau

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Abstract

An enterovirus (EV) strain, designated as SWUN-AB001, was isolated in the Qinghai-Tibetan Plateau from a yak with severe diarrheal disease. The complete genome of strain SWUN-AB001 was 7,382 bp in length and shared 35.1–68.5% nt identities with bovine EVs belonging to a candidate new type EV-F7. Using the sequence difference values in the VP1 gene as a criterion for demarcating a new serotype/genotype in the Enterovirus genus, strain SWUN-AB001 had only a 71.1% nt and a 79.2% aa identity, in the VP1 region, with the most closely matched EV, further indicating that a new type of EV had been identified. Phylogenetic analysis of the nt sequence of the viral polyprotein and of VP1 genes demonstrated that the virus fell within the EV-F cluster, but was located in a unique lineage. Furthermore, a large-scale surveillance study indicated that the prevalence of this EV in yaks was 31.05% (95% CI = 25.5–37.6%) in 235 animals with diarrhea and 24.13% (95% CI = 17.4–32.4%) in 116 healthy yaks. However, there was no significant difference in virus prevalence between diarrheal and healthy samples. Interestingly, in the Tibet region, diarrheal feces had a higher incidence of EVs than feces of healthy yaks (odd ratios = 6.03, 95% CI = 1.93–18.86), indicating that the incidence of EV was potentially correlated with the clinical symptom of diarrhea in yaks.

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Acknowledgements

This work was supported by funding from: the 13th Five-Year Plan National Key R & D Program (Grant Number 2016YFD0500907), the Sichuan Provincial Science and Technology Department Program for Young Scholars (Grant Number 2014JQ0044), the Innovative Research Team Program of Department of Education of Sichuan Province (Grant Number 13TD0057), and the Fundamental Research Funds for the Central Universities, Southwest University for Nationalities (Grant Number 2016NGJPY10).

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Authors and Affiliations

  1. College of Life Science and Technology, Southwest University for Nationalities, No. 16, South 4th Section 1st Ring Road, Chengdu, 610041, China

    Huan He, Cheng Tang, Xinnuo Chen, Hua Yue, Yupeng Ren, Yan Liu & Bin Zhang

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  1. Huan He
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  2. Cheng Tang
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  3. Xinnuo Chen
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  4. Hua Yue
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  5. Yupeng Ren
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  6. Yan Liu
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  7. Bin Zhang
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Correspondence to Cheng Tang or Bin Zhang.

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He, H., Tang, C., Chen, X. et al. Isolation and characterization of a new enterovirus F in yak feces in the Qinghai-Tibetan Plateau. Arch Virol 162, 523–527 (2017). https://doi.org/10.1007/s00705-016-3119-x

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