Skip to main content

Advertisement

SpringerLink
Log in

Molecular characterization of a new human echovirus 11 isolate associated with severe hand, foot and mouth disease in Yunnan, China, in 2010

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Human echovirus 11 (E-11), a member of the species Enterovirus B, frequently causes aseptic meningitis and hand, foot and mouth disease (HFMD). We determined the complete genome sequence of strain 520K/YN/CHN/2010, isolated from a subject with HFMD and aseptic meningitis in Yunnan Province, China, in 2010. The strain shared 78.8 % and 81.1 % nucleotide sequence similarity with prototype strain Gregory in the complete VP1 gene and the complete genome, respectively. Only the VP2–VP3–VP1 genome region of 520K/YN/CHN/2010 was similar to that of the E-11 strain; the other genome regions were most similar to those of other members of the species Enterovirus B. Using phylogenetic analysis and sequence comparisons of the complete VP1 gene, E-11 strains could be divided into five genogroups, and the 520K/YN/CHN/2010 strain was found to belong to genogroup A. Recombination analysis showed evidence of recombination with other member of the species Enterovirus B, especially the E-9 strain MSH/KM812/2010. Persistent surveillance of HFMD pathogens might help predict potential emerging viruses and related disease outbreaks.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  1. Lee HY, Chen CJ, Huang YC, Li WC, Chiu CH, Huang CG, Tsao KC, Wu CT, Lin TY (2010) Clinical features of echovirus 6 and 9 infections in children. J Clin Virol 49:175–179

    Article  PubMed  Google Scholar 

  2. Okonko IO, Adebiyi AA, Ogah OS, Adu FD (2013) Enteroviruses as a possible cause of hypertension, dilated cardiomyopathy (DCM) and hypertensive heart failure (HHF) in South western Nigeria. Afr Health Sci 13:1098–1106

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  3. Centers for Disease Control and Prevention (CDC) (2010) Nonpolio enterovirus and human parechovirus surveillance—United States, 2006–2008. MMWR Morb Mortal Wkly Rep 59:1577–1580

    Google Scholar 

  4. Oyero OG, Adu FD, Ayukekbong JA (2014) Molecular characterization of diverse species enterovirus-B types from children with acute flaccid paralysis and asymptomatic children in Nigeria. Virus Res 189:189–193

    Article  CAS  PubMed  Google Scholar 

  5. Tan CY, Ninove L, Gaudart J, Nougairede A, Zandotti C, Thirion-Perrier L, Charrel RN, de Lamballerie X (2011) A retrospective overview of enterovirus infection diagnosis and molecular epidemiology in the public hospitals of Marseille, France (1985–2005). PLoS ONE 6:e18022

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  6. Kim HJ, Kang B, Hwang S, Hong J, Kim K, Cheon DS (2012) Epidemics of viral meningitis caused by echovirus 6 and 30 in Korea in 2008. Virol J 9:38

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  7. Papadakis G, Chibo D, Druce J, Catton M, Birch C (2014) Detection and genotyping of enteroviruses in cerebrospinal fluid in patients in Victoria, Australia, 2007–2013. J Med Virol 86:1609–1613

    Article  CAS  PubMed  Google Scholar 

  8. Tao Z, Wang H, Li Y, Liu G, Xu A, Lin X, Song L, Ji F, Wang S, Cui N, Song Y (2014) Molecular epidemiology of human enterovirus associated with aseptic meningitis in Shandong Province, China, 2006–2012. PLoS ONE 9:e89766

    Article  PubMed Central  PubMed  Google Scholar 

  9. Oberste MS, Nix WA, Kilpatrick DR, Flemister MR, Pallansch MA (2003) Molecular epidemiology and type-specific detection of echovirus 11 isolates from the Americas, Europe, Africa, Australia, southern Asia and the Middle East. Virus Res 91:241–248

    Article  CAS  PubMed  Google Scholar 

  10. Somekh E, Shohat T, Handsher R, Serour F (2001) An outbreak of echovirus 11 in a children’s home. Epidemiol Infect 126:441–444

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  11. Guan HY, Yang MJ, Liu LZ, Wang J, Yang GL, Zhang W, Ma XJ, Wang CR (2014) Pathogenic spectrum of enteroviruses associated with hand, foot and mouth disease by a GeXP-based multiplex reverse transcription-PCR assay in Jinan, China, 2009–2012. Bing Du Xue Bao 30:567–571

    CAS  PubMed  Google Scholar 

  12. Apisarnthanarak A, Kitphati R, Pongsuwann Y, Tacharoenmueng R, Mundy LM (2005) Echovirus type 11: outbreak of hand-foot-and-mouth disease in a Thai hospital nursery. Clin Infect Dis 41:1361–1362

    Article  PubMed  Google Scholar 

  13. Cui A, Xu C, Tan X, Zhang Y, Zhu Z, Mao N, Lu Y, Xu W (2013) The development and application of the two real-time RT-PCR assays to detect the pathogen of HFMD. PLoS ONE 8:e61451

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  14. Lukashev AN, Lashkevich VA, Koroleva GA, Karganova GG (2002) Phylogenetic and serological characterization of echovirus 11 and echovirus 19 strains causing uveitis. Arch Virol 147:131–142

    Article  CAS  PubMed  Google Scholar 

  15. Lukashev AN, Lashkevich VA, Koroleva GA, Ilonen J, Hinkkanen AE (2004) Recombination in uveitis-causing enterovirus strains. J Gen Virol 85:463–470

    Article  CAS  PubMed  Google Scholar 

  16. El-Sageyer MM, Szendroi A, Hutter E, Uj M, Szucs G, Mezey I, Toth I, Katai A, Kapiller Z, Pall G, Petras G, Szalay E, Mihaly I, Gourova S, Berencsi G (1998) Characterisation of an echovirus type 11’ (prime) epidemic strain causing haemorrhagic syndrome in newborn babies in Hungary. Acta Virol 42:157–166

    CAS  PubMed  Google Scholar 

  17. Fares W, Rezig D, Seghier M, Ben YA, Touzi H, Triki H (2011) Phylogenetic analysis of complete VP1 sequences of echoviruses 11 and 6: high genetic diversity and circulation of genotypes with a wide geographical and temporal range. J Med Microbiol 60:1017–1025

    Article  PubMed  Google Scholar 

  18. Rezaikin AV, Novoselov AV, Sergeev AG, Fadeyev FA, Lebedev SV (2009) Two clusters of mutations map distinct receptor-binding sites of echovirus 11 for the decay-accelerating factor (CD55) and for canyon-binding receptors. Virus Res 145:74–79

    Article  CAS  PubMed  Google Scholar 

  19. Novoselov AV, Rezaykin AV, Sergeev AG, Fadeyev FA, Grigoryeva JV, Sokolova ZI (2012) A single amino acid substitution controls DAF-dependent phenotype of echovirus 11 in rhabdomyosarcoma cells. Virus Res 166:87–96

    Article  CAS  PubMed  Google Scholar 

  20. Savolainen-Kopra C, Al-Hello H, Paananen A, Blomqvist S, Klemola P, Sobotova Z, Roivainen M (2009) Molecular epidemiology and dual serotype specificity detection of echovirus 11 strains in Finland. Virus Res 139:32–38

    Article  CAS  PubMed  Google Scholar 

  21. Benschop KS, Wildenbeest JG, Koen G, Minnaar RP, van Hemert FJ, Westerhuis BM, Pajkrt D, van den Broek PJ, Vossen AC, Wolthers KC (2015) Genetic and antigenic structural characterization for resistance of echovirus 11 to pleconaril in an immunocompromised patient. J Gen Virol 96:571–579

    Article  CAS  PubMed  Google Scholar 

  22. Chevaliez S, Szendroi A, Caro V, Balanant J, Guillot S, Berencsi G, Delpeyroux F (2004) Molecular comparison of echovirus 11 strains circulating in Europe during an epidemic of multisystem hemorrhagic disease of infants indicates that evolution generally occurs by recombination. Virology 325:56–70

    Article  CAS  PubMed  Google Scholar 

  23. Lukashev AN, Lashkevich VA, Ivanova OE, Koroleva GA, Hinkkanen AE, Ilonen J (2005) Recombination in circulating human enterovirus B: independent evolution of structural and non-structural genome regions. J Gen Virol 86:3281–3290

    Article  CAS  PubMed  Google Scholar 

  24. Chan YF, Sam IC, AbuBakar S (2010) Phylogenetic designation of enterovirus 71 genotypes and subgenotypes using complete genome sequences. Infect Genet Evol 10:404–412

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the Basic Research Projects of Yunnan Province, China (2013FZ136).

Author information

Author notes

    Authors and Affiliations

    1. Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College (CAMS and PUMC), 935 Jiao Ling Road, Kunming, 650118, Yunnan, People’s Republic of China

      Ting Su, Yan Zhou, Yanju Zhu, Zhengling Liu, Shunqiao Yang, Zexu Yu, Chen Guo & Shaohui Ma

    2. Yunnan Key Laboratory of Vaccine Research Development on Severe Infectious Disease, Kunming, 650118, People’s Republic of China

      Ting Su, Yan Zhou, Yanju Zhu, Zhengling Liu, Shunqiao Yang, Zexu Yu, Chen Guo & Shaohui Ma

    3. Department of Medical Genetics and Cell Biology, Kunming Medical University, Kunming, People’s Republic of China

      Fang Yang

    Authors
    1. Ting Su
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Yan Zhou
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. Yanju Zhu
      View author publications

      You can also search for this author in PubMed Google Scholar

    4. Zhengling Liu
      View author publications

      You can also search for this author in PubMed Google Scholar

    5. Fang Yang
      View author publications

      You can also search for this author in PubMed Google Scholar

    6. Shunqiao Yang
      View author publications

      You can also search for this author in PubMed Google Scholar

    7. Zexu Yu
      View author publications

      You can also search for this author in PubMed Google Scholar

    8. Chen Guo
      View author publications

      You can also search for this author in PubMed Google Scholar

    9. Shaohui Ma
      View author publications

      You can also search for this author in PubMed Google Scholar

    Corresponding author

    Correspondence to Shaohui Ma.

    Additional information

    T. Su and Y. Zhou have contributed equally to this study.

    Electronic supplementary material

    Below is the link to the electronic supplementary material.

    Supplementary material 1 (DOC 60 kb)

    Rights and permissions

    Reprints and permissions

    About this article

    Check for updates. Verify currency and authenticity via CrossMark

    Cite this article

    Su, T., Zhou, Y., Zhu, Y. et al. Molecular characterization of a new human echovirus 11 isolate associated with severe hand, foot and mouth disease in Yunnan, China, in 2010. Arch Virol 160, 2343–2347 (2015). https://doi.org/10.1007/s00705-015-2496-x

    Download citation

    • Received:

    • Accepted:

    • Published:

    • Issue Date:

    • DOI: https://doi.org/10.1007/s00705-015-2496-x

    Keywords

    Search

    Navigation