Skip to main content

Advertisement

SpringerLink
Log in

In vitro antiviral activity of germacrone against porcine parvovirus

  • Original Article
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Porcine parvovirus (PPV) infections can lead to significant losses to the swine industry by causing reproductive failure in pigs. Germacrone has been reported to efficiently suppress the replication of influenza virus. In this report, the antiviral activity of germacrone on PPV in swine testis (ST) cells was investigated. Here, we show for the first time that germacrone protects cells from PPV infection and suppresses the synthesis of viral mRNA and protein. Furthermore, we show that germacrone inhibits PPV replication at an early stage in a dose-dependent manner. These findings suggest that germacrone is a potential candidate for anti-PPV therapy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Allan GM, Kennedy S, McNeilly F, Foster JC, Ellis JA, Krakowka SJ, Meehan BM, Adair BM (1999) Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus. J Comp Pathol 121:1–11

    Article  CAS  PubMed  Google Scholar 

  2. Cartwright SF, Lucas M, Huck RA (1969) A small haemagglutinating porcine DNA virus. I. Isolation and properties. J Comp Pathol 79:371–377

    Article  CAS  PubMed  Google Scholar 

  3. Chen Y, Yan H, Zheng H, Shi Y, Sun L, Wang C, Sun J (2015) Antiviral effect of lithium chloride on cell infection by porcine parvovirus. Arch Virol 160:1015–1020

    Article  CAS  PubMed  Google Scholar 

  4. Cheung AK, Wu G, Wang D, Bayles DO, Lager KM, Vincent AL (2010) Identification and molecular cloning of a novel porcine parvovirus. Arch Virol 155:801–806

    Article  CAS  PubMed  Google Scholar 

  5. Cho W, Nam JW, Kang HJ, Windono T, Seo EK, Lee KT (2009) Zedoarondiol isolated from the rhizoma of Curcuma heyneana is involved in the inhibition of iNOS, COX-2 and pro-inflammatory cytokines via the downregulation of NF-kappa B pathway in LPS-stimulated murine macrophages. Int Immunopharmacol 9:1049–1057

    Article  CAS  PubMed  Google Scholar 

  6. Cui J, Wang X, Ren Y, Cui S, Li G, Ren X (2012) Genome sequence of Chinese porcine parvovirus strain PPV2010. J Virol 86:2379

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  7. Ellis JA, Bratanich A, Clark EG, Allan G, Meehan B, Haines DM, Harding J, West KH, Krakowka S, Konoby C, Hassard L, Martin K, McNeilly F (2000) Coinfection by porcine circoviruses and porcine parvovirus in pigs with naturally acquired postweaning multisystemic wasting syndrome. J Vet Diagn Invest 12:21–27

    Article  CAS  PubMed  Google Scholar 

  8. Jozwik A, Manteufel J, Selbitz HJ, Truyen U (2009) Vaccination against porcine parvovirus protects against disease, but does not prevent infection and virus shedding after challenge infection with a heterologous virus strain. J Gen Virol 90:2437–2441

    Article  CAS  PubMed  Google Scholar 

  9. Li Y, Wo JM, Liu Q, Li X, Martin RC (2009) Chemoprotective effects of Curcuma aromatica on esophageal carcinogenesis. Ann Surg Oncol 16:515–523

    Article  PubMed  Google Scholar 

  10. Liao QJ, Qian ZX, Liu R, An LW, Chen XL (2013) Germacrone inhibits early stages of influenza virus infection. Antivir Res 100:578–588

    Article  CAS  PubMed  Google Scholar 

  11. Liu YY, Wang W, Fang B, Ma FY, Zheng Q, Deng PY, Zhao SS, Chen MJ, Yang GX, He GY (2013) Anti-tumor effect of germacrone on human hepatoma cell lines through inducing G2/M cell cycle arrest and promoting apoptosis. Eur J Pharmacol 698:95–102

    Article  CAS  PubMed  Google Scholar 

  12. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25:402–408

    Article  CAS  PubMed  Google Scholar 

  13. Mahnel H, Mayr A, Bibrack B (1966) Multiplication of swine fever virus with cytopathogenic effect in piglet testis culture. Zentralblatt fur Veterinarmedizin Reihe B (J Vet Med Series B) 13:250–259

    Article  CAS  Google Scholar 

  14. Mengeling WL (1972) Porcine parvovirus: properties and prevalence of a strain isolated in the United States. Am J Vet Res 33:2239–2248

    CAS  PubMed  Google Scholar 

  15. Reed LJ, Muench H (1938) A simple method of estimating fifty per cent endpoints. Am J Epidemiol 27:493–497

    Google Scholar 

  16. van Leengoed LA, Vos J, Gruys E, Rondhuis P, Brand A (1983) Porcine Parvovirus infection: review and diagnosis in a sow herd with reproductive failure. Vet Q 5:131–141

    Article  PubMed  Google Scholar 

  17. Wu R, Wen Y, Huang X, Wen X, Yan Q, Huang Y, Ma X, Cao S (2014) First complete genomic characterization of a porcine parvovirus 5 isolate from China. Arch Virol 159:1533–1536

    Article  CAS  PubMed  Google Scholar 

  18. Xiao X, Zhao Y, Yuan H, Xia W, Zhao J, Wang X (2002) Study on the effect of Rhizoma Curcuma Longa on gastrin receptor. Zhong Yao Cai (J Chin Med Mater) 25:184–185

  19. Zeeuw EJ, Leinecker N, Herwig V, Selbitz HJ, Truyen U (2007) Study of the virulence and cross-neutralization capability of recent porcine parvovirus field isolates and vaccine viruses in experimentally infected pregnant gilts. J Gen Virol 88:420–427

    Article  CAS  PubMed  Google Scholar 

  20. Zhong ZF, Chen XP, Zhou KY, Wu T, Cui LA, Wang YT (2010) Rhizoma curcuma exerts synthetic effect of anti-breast cancer through suppressing cell proliferation, metastasis and angiogenesis in vitro. Int J Mol Med 26:S39–S139

    Google Scholar 

  21. Zhong ZF, Chen XP, Tan W, Xu ZT, Zhou KY, Wu T, Cui L, Wang YT (2011) Germacrone inhibits the proliferation of breast cancer cell lines by inducing cell cycle arrest and promoting apoptosis. Eur J Pharmacol 667:50–55

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Guangdong Provincial Key Lab of Agro-animal Genomics and Molecular Breeding, College of Animal Science, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, Guangdong, 510642, China

    Ye Chen, Yiren Jiao, Lianjie Hou, Yuzhen Shi, Ting Gu, Pei Zhou, Zhongyuan Shi, Lulu Xu & Chong Wang

  2. Department of Parasitology, Harbin Medical University, 157 Baojian Road, Harbin, Heilongjiang, 150081, China

    Yunxia Dong

Authors
  1. Ye Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yunxia Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yiren Jiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lianjie Hou
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yuzhen Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ting Gu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Pei Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Zhongyuan Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Lulu Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Chong Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Yunxia Dong or Chong Wang.

Additional information

Y. Chen, Y. Dong and Y. Jiao are co-first authors.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chen, Y., Dong, Y., Jiao, Y. et al. In vitro antiviral activity of germacrone against porcine parvovirus. Arch Virol 160, 1415–1420 (2015). https://doi.org/10.1007/s00705-015-2393-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-015-2393-3

Keywords

Search

Navigation