Abstract
Botryosphaeria dothidea is a pathogen that causes many important diseases of deciduous fruit trees worldwide. In this study, a double-stranded (ds) RNA of 5322 base pairs (bp) in length from strain GY25 of B. dothidea was isolated and sequenced. The dsRNA potentially contained two overlapping open reading frames (ORFs). The predicted gene product of ORF1 had the highest amino acid sequence similarity, 37 % (E-value, 8e−101), to the capsid protein of Rosellinia necatrix victorivirus 1 (RnV1), and ORF2 had the highest similarity, 44 % (E-value, 0.0), to the RNA-directed RNA polymerase of Beauveria bassiana victorivirus NZL/1980(BbV-NZL/1980). Both of these viruses are members of the genus Victorivirus in the family Totiviridae. There was a small ORF at the 5′ terminus whose predicted gene product did not show homology to any known proteins. The dsRNA from B. dothidea strain GY25 appears to be a genomic component of a victorivirus, which we have tentatively named “Botryosphaeria dothidea victorivirus 1” (BdV1). This is the first full-length genome sequence of a putative victorivirus infecting B. dothidea.
Similar content being viewed by others
References
Bruenn JA (2003) A structural and primary sequence comparison of the viral RNA dependent RNA polymerases. Nucleic Acids Res 31:1821–1829
De Paulo JJ, Powell CA (1995) Extraction of double-stranded RNA from plant tissues without the use of organic solvents. Plant Dis 79:246–248
Ghabrial SA, Suzuki N (2009) Viruses of plant pathogenic fungi. Annu Rev Phytopathol 47:353–384
Ghabrial SA, Nibert ML (2009) Victorivirus, a new genus of fungal viruses in the family Totiviridae. Arch Virol 154:373–379
Herrero N, Duenas E, Quesada-Moraga E, Zabalgogeazcoa I (2012) Prevalence and diversity of viruses in the entomopathogenic fungus Beauveria bassiana. Appl Environ Microbiol 78:8523–8530
King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) (2011) Virus taxonomy: ninth report of international committee on taxonomy of viruses. Elsevier Academic Press, London
Kozlakidis Z, Herrero N, Coutts RHA (2013) The complete nucleotide sequence of a totivirus from Aspergillus foetidus. Arch Virol 158:263–266
Lambden PR, Cooke SJ, Caul EO, Clarke IN (1992) Cloning of noncultivatable human rotavirus by single primer amplification. J Virol 66:1817–1822
Liu HQ, Fu YP, Jiang DH, Li GQ, Xie J, Peng YL, Yi XH, Ghabrial SA (2009) A novel mycovirus that is related to the human pathogen hepatitis E virus and rubi-like viruses. J Virol 83:1981–1991
Pearson MN, Beever RE, Boine B, Arthur K (2009) Mycoviruses of filamentous fungi and their relevance to plant pathology. Mol Plant Pathol 10:115–128
Slippers B, Smit WA, Crous PW, Coutinho TA, Wingfield BD, Wingfield MJ (2007) Taxonomy, phylogeny and identification of Botryosphaeriaceae associated with pome and stone fruit trees in South Africa and other regions of the world. Plant Pathology 56:128–139
Stuart KD, Weeks R, Guilbride L, Myler PJ (1992) Molecular organization of Leishmania RNA virus 1. Proc Natl Acad Sci USA 89:8596–8600
Wang LP, Jiang JJ, Wang YF, Hong N, Zhang FP, Xu WX, Wang GP (2014) Hypovirulence of the phytopathogenic fungus Botryosphaeria dothidea: association with a co-infecting chrysovirus and a partitivirus. J Virol 88:7517–7527
Xie JT, Jiang DH (2014) New Insights into mycoviruses and exploration for the biological control of crop fungal diseases. Annu Rev Phytopathol 52:45–68
Yu X, Li B, Fu YP, Xie JT, Cheng JS, Ghabrial SA, Li GX, Yi XH, Jiang DH (2013) Extracellular transmission of a DNA mycovirus and its use as a natural fungicide. Proc Natl Acad Sci USA 588:1452–1457
Zhai LF, Zhang MX, Lv G, Chen XR, Jia NN, Hong N, Wang GP (2014) Biological and molecular characterization of four Botryosphaeria species isolated from pear plants showing stem wart and stem canker in China. Plant Dis 98:716–726
Acknowledgments
This study was supported financially by the Chinese Ministry of Agriculture, Industry Technology Research Project (grant nos. 201203034-02 and 200903004-05) and the earmarked fund for Pear Modern Agro-Industry Technology Research System (CARS-29-10).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zhai, L., Hong, N., Zhang, M. et al. Complete dsRNA sequence of a novel victorivirus isolated from the pear stem wart fungus Botryosphaeria dothidea . Arch Virol 160, 613–616 (2015). https://doi.org/10.1007/s00705-014-2285-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-014-2285-y