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Analysis of the complete DNA sequence of rice tungro bacilliform virus from southern India indicates it to be a product of recombination

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Abstract

The complete nucleotide sequence of an isolate of rice tungro bacilliform virus (RTBV), collected from Kanyakumari, India, where RTBV was reported recently for the first time, has been analyzed. Sequence comparison revealed that the RTBV isolate from Kanyakumari (RTBV-KK) has a high degree of identity to the two previously reported RTBV sequences from India, RTBV-AP and RTBV-WB, which had been collected from field locations about 10 years ago and 1000–2000 km away from the collection site of RTBV-KK. Most of the sequence domains reported previously in other RTBV isolates were found to be conserved in RTBV-KK. Closer inspection revealed RTBV-KK to be a possible recombinant between RTBV-AP and RTBV-WB in the genomic region encompassing the coat protein gene.

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Acknowledgments

SS is indebted to the Council of Scientific and Industrial Research, New Delhi, for research fellowship during this work. This work was funded by the Department of Biotechnology, Government of India.

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Authors and Affiliations

  1. Department of Plant Molecular Biology, University of Delhi, South Campus, Benito Juarez Road, New Delhi, 110021, India

    S. Sharma & I. Dasgupta

  2. Department of Rice, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Lawley Road, Coimbatore, 641003, Tamil Nadu, India

    R. Rabindran & S. Robin

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  1. S. Sharma
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  2. R. Rabindran
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  3. S. Robin
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  4. I. Dasgupta
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Corresponding author

Correspondence to I. Dasgupta.

Additional information

Nucleotide sequence data mentioned in the manuscript are available in the GenBank sequence database under accession number HQ385226.

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Supplementary material 1 (DOC 33 kb)

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Sharma, S., Rabindran, R., Robin, S. et al. Analysis of the complete DNA sequence of rice tungro bacilliform virus from southern India indicates it to be a product of recombination. Arch Virol 156, 2257–2262 (2011). https://doi.org/10.1007/s00705-011-1092-y

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  • DOI: https://doi.org/10.1007/s00705-011-1092-y

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