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Detection of Dobrava-Belgrade hantavirus using recombinant-nucleocapsid-based enzyme-linked immunosorbent assay and SYBR Green-based real-time reverse transcriptase-polymerase chain reaction

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Abstract

Dobrava (DOBV) hantaviruses belong to the genus Hantavirus, family Bunyaviridae, and are carried by yellow-necked and striped field mice. The goal of this study was to detect DOBV using serological and genetic methods in Apodemus rodents in Hungary and in northern Croatia. During the study period, a total of 125 Apodemus sp. (67 A. agrarius, 58 A. flavicollis) were tested for the presence of hantaviruses, and 21 rodents (17%) were positive by rRT-PCR and/or ELISA. We conclude that the prevalence of DOBV is much higher than previously anticipated. The simultaneous use of molecular and serological techniques provides a highly reliable way to detect hantavirus infections.

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Acknowledgment

This study was supported by the Hungarian Scientific Research Fund (OTKA; PD77977) and Science, Please! Research Team on Innovation (SROP-4.2.2/08/1/2008-0011) project. The study was performed during the period of the “Bolyai János” Research Fellowship of Ferenc Jakab and Krisztián Bányai. We also would like to give special thanks to Dr. Emőke Ferenczi and Kaposi Tamásné (National Center for Epidemiology, Department of Viral Diagnostics, Reference Laboratory of Viral Zoonozes, Budapest, Hungary). We thank Tibor Pál for his comments and assistance in editing of the manuscript.

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Authors and Affiliations

  1. Virological Research Group, Faculty of Sciences, Institute of Biology, University of Pécs, Ifjúság út 6, 7624, Pecs, Hungary

    Viktória Németh, Mónika Madai, Alexandra Maráczi, Bálint Bérczi, Miklós Oldal & Ferenc Jakab

  2. Virological Laboratory, Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pecs, Hungary

    Ferenc Jakab

  3. Department of Animal Ecology, Faculty of Sciences, Institute of Biology, University of Pécs, Pecs, Hungary

    Győző Horváth

  4. Department of Medical Genetics, Medical School, University of Pécs, Pecs, Hungary

    Péter Kisfali

  5. Veterinary Medical Research Institute Hungarian Academy of Sciences, Budapest, Hungary

    Krisztián Bányai

Authors
  1. Viktória Németh
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  2. Mónika Madai
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  3. Alexandra Maráczi
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  4. Bálint Bérczi
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  5. Győző Horváth
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  6. Miklós Oldal
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  7. Péter Kisfali
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  8. Krisztián Bányai
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  9. Ferenc Jakab
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Correspondence to Ferenc Jakab.

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Németh, V., Madai, M., Maráczi, A. et al. Detection of Dobrava-Belgrade hantavirus using recombinant-nucleocapsid-based enzyme-linked immunosorbent assay and SYBR Green-based real-time reverse transcriptase-polymerase chain reaction. Arch Virol 156, 1655–1660 (2011). https://doi.org/10.1007/s00705-011-1013-0

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  • DOI: https://doi.org/10.1007/s00705-011-1013-0

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