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Molecular and biological comparison of two Tomato yellow ring virus (TYRV) isolates: challenging the Tospovirus species concept

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Summary.

Two strains of Tomato yellow ring virus (TYRV, genus Tospovirus), one from tomato (referred to as TYRV-t) and the other from soybean and potato (denoted TYRV-s), collected from different geographical regions in Iran, were compared. Their genomic S RNA segments differed in size by 55 nucleotides. Comparison of the S RNA intergenic regions revealed the absence of a stretch of 115 nucleotides within the S RNA segment of TYRV-s and, conversely, of 56 nts in that of TYRV-t, apparently a stable genetic difference as it was also found in another isolate of TYRV-s collected from potato. Sequence comparison of the N protein ORFs revealed an identity of 92% between the N proteins of both strains, and the observed strong cross-reaction of TYRV-s in DAS-ELISA with a polyclonal antiserum directed against the TYRV-t N protein confirmed this high identity. Host range analysis revealed several differences, e.g. TYRV-s, but not TYRV-t, being able to systemically infect Nicotiana species, and TYRV-s being localised in tomato. The observed molecular and biological differences of both viruses call into question the currently used criteria for Tospovirus species demarcation.

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Authors and Affiliations

  1. Laboratory of Virology, Wageningen University, Wageningen, The Netherlands

    A. Hassani-Mehraban, J. Saaijer, D. Peters, R. Goldbach & R. Kormelink

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  1. A. Hassani-Mehraban
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  2. J. Saaijer
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  3. D. Peters
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  4. R. Goldbach
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  5. R. Kormelink
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Hassani-Mehraban, A., Saaijer, J., Peters, D. et al. Molecular and biological comparison of two Tomato yellow ring virus (TYRV) isolates: challenging the Tospovirus species concept. Arch Virol 152, 85–96 (2007). https://doi.org/10.1007/s00705-006-0827-7

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  • DOI: https://doi.org/10.1007/s00705-006-0827-7

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