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Fluorometric determination of the antibiotic kanamycin by aptamer-induced FRET quenching and recovery between MoS2 nanosheets and carbon dots

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Abstract

A rapid and sensitive aptamer-based assay is described for kanamycin, a veterinary antibiotic with neurotoxic side effects. It is based on a novel FRET pair consisting of fluorescent carbon dots and layered MoS2. This donor-acceptor pair (operated at excitation/emission wavelengths of 380/440 nm) shows fluorescence recovery efficiencies reaching 93 %. By taking advantages of aptamer-induced fluorescence quenching and recovery, kanamycin can be quantified in the of 4–25 μM concentration range, with a detection limit of 1.1 μM. The method displays good specificity and was applied to the determination of kanamycin in spiked milk where it gave recoveries ranging from 85 % to 102 %, demonstrating that the method serves as a promising tool for the rapid detection of kanamycin in milk and other animal-derived foodstuff.

A fluorometric aptasensor was developed for the determination of kanamycin. It is based on a novel FRET pair of carbon dots and layered MoS2. The fluorescence recovery efficiency reached 93 % with a good sensitivity, specificity and recoveries in spiked milk.

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Acknowledgments

This work is supported by the National Science & Technology Pillar Program of China (No. 2014BAD13B05) and the Yangling Agricultural Hi-tech Industries Demonstration Zone (2014NY-35).

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Authors and Affiliations

  1. Ministry of Agriculture, China Animal Disease Control Center, No. 20 Maizidian Street, Beijing, 100125, China

    Yashan Wang, Yongjun Liu, Yaping Tian, Ruinan Wang, Yanbin Jiang & Dongjun Hou

  2. Institute of Chemistry, Chinese Academy of Sciences, Bei Yi Jie 2, Zhong Guan Cun, Beijing, 100190, China

    Tiancong Ma

  3. College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Shuyue Ma & Jianlong Wang

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  1. Yashan Wang
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  2. Tiancong Ma
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  3. Shuyue Ma
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Correspondence to Dongjun Hou or Jianlong Wang.

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Wang, Y., Ma, T., Ma, S. et al. Fluorometric determination of the antibiotic kanamycin by aptamer-induced FRET quenching and recovery between MoS2 nanosheets and carbon dots. Microchim Acta 184, 203–210 (2017). https://doi.org/10.1007/s00604-016-2011-4

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  • DOI: https://doi.org/10.1007/s00604-016-2011-4

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