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A flexible electrochemical micro lab-on-chip: application to the detection of interleukin-10

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Abstract

We report on a simple method to manufacture an electrochemical micro lab-on-chip (μLoC), for the detection of interleukin-10 (IL-10) cytokine of patients suffering from heart failure (HF). To monitor the critical levels of inflammation, a μLoC containing eight gold microelectrodes based on a polyimide (PI) substrate was fabricated. The microelectrodes were manufactured on PI by a combination of soft lithographical tools. To produce an operational μLoC, a microfluidic system fabricated in polydimethylsiloxane was sealed onto the PI substrate through silane reagent (3-aminopropyl)triethoxysilane. Cyclic voltammetry was applied as the characterization technique for the gold microelectrode surface properties. Finally, electrochemical characterization of the μLoC was determined by electrochemical impedance spectroscopy for the quantification of IL-10. These were detected within the range of 1–15 pg.mL−1. The time and cost of fabrication for this μLoC was very low when compared to those that have been fabricated by conventional lithography.

A fully integrated micro Lab-on-Chip (μLoC) based on flexible and biocompatible polymers for Interleukin-10. detection

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Authors and Affiliations

  1. Université de Lyon, Université de Claude Bernard Lyon 1 UMR 5280, 5 rue de la Doua, Institut des Sciences Analytiques (ISA), 69100, Villeurbanne, France

    Abdoullatif Baraket, Michael Lee, Nadia Zine & Abdelhamid Errachid

  2. Université du Maine LAUM UMR CNRS 6613, Bd. Olivier Messiaen, 72085, MANS Cedex9, LE, France

    Nourdin Yaakoubi

  3. CentroNacional de Microelectróica (IMB-CSIC), Campus UAB, 08193 Bellaterra, Barcelona, Spain

    Joan Bausells

Authors
  1. Abdoullatif Baraket
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  2. Michael Lee
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  3. Nadia Zine
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  4. Nourdin Yaakoubi
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  5. Joan Bausells
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  6. Abdelhamid Errachid
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Correspondence to Abdelhamid Errachid.

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Baraket, A., Lee, M., Zine, N. et al. A flexible electrochemical micro lab-on-chip: application to the detection of interleukin-10. Microchim Acta 183, 2155–2162 (2016). https://doi.org/10.1007/s00604-016-1847-y

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  • DOI: https://doi.org/10.1007/s00604-016-1847-y

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