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Recognition and determination of bovine hemoglobin using a gold electrode modified with gold nanoparticles and molecularly imprinted self-polymerized dopamine

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Abstract

A molecularly imprinted polymer (MIP) was prepared by self-polymerization of dopamine in the presence of bovine hemoglobin (BHb) and then deposited on the surface of an electrode modified with gold nanoparticles (AuNPs). Scanning electron microscopy, cyclic voltammetry, and differential pulse voltammetry were employed to characterize the modified electrode using the hexacyanoferrate redox system as an electroactive probe. The effects of BHb concentration, dopamine concentration, and polymerization time were optimized. Under optimized conditions, the modified electrode selectively recognizes BHb even in the presence of other proteins. The peak current for hexacyanoferrate, typically measured at + 0.17 V (vs. SCE), depends on the concentration of BHb in the 1.0 × 10−11 to 1.0 × 10−2 mg mL−1 range. Due to the ease of preparation and tight adherence of polydopamine to various support materials, the present strategy conceivably also provides a platform for the recognition and detection of other proteins.

Gold nanoparticles and molecularly imprinted self-polymerization dopamine were modified on gold electrode surface to recognize and determine bovine hemoglobin. Under the optimized conditions, the modified electrode showed specific adsorption, selective recognition, and sensitive detection of bovine hemoglobin.

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Acknowledgments

We greatly appreciate the support of the National Natural Science Foundation of China for young program (21005002), Anhui Provincial Natural Science Foundation for Young Program (11040606Q35), Anhui University Provincial Natural Science Foundation Key program (KJ2010A138).

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Authors and Affiliations

  1. College of Chemistry and Materials Science, Anhui Key Laboratory of Chemo-Biosensing, Anhui Key Laboratory of Functional Molecular Solids, Anhui Normal University, Wuhu, 241000, Peoples Republic of China

    Lu Li, Limei Fan, Yunlong Dai & Xianwen Kan

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  1. Lu Li
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  2. Limei Fan
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  3. Yunlong Dai
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  4. Xianwen Kan
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Correspondence to Xianwen Kan.

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Li, L., Fan, L., Dai, Y. et al. Recognition and determination of bovine hemoglobin using a gold electrode modified with gold nanoparticles and molecularly imprinted self-polymerized dopamine. Microchim Acta 182, 2477–2483 (2015). https://doi.org/10.1007/s00604-015-1594-5

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  • DOI: https://doi.org/10.1007/s00604-015-1594-5

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