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Aptasensor for adenosine triphosphate based on electrode–supported lipid bilayer membrane

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Abstract

We have developed an aptasensor for adenosine triphosphate (ATP) based on an electrode-supported lipid bilayer membrane. The assay is based on a conformational change that is induced after binding the target which modulates the electron transfer rate in the conductive path. The method is highly sensitive, stable, and repeatable. The detection limit for ATP is 50 nM, and the dynamic range extends to 3.2 μM, which covers the concentration range of ATP in cell lysates (from 0.1 to 1 μM). The method also holds promise in that it may be transferred to submicro- or nano-scale electrodes so to enable intracellular monitoring of ATP.

An aptasensor for adenosine triphosphate based on an electrode-supported lipid bilayer membrane in principle of target-binding induced conformational change to modulate the electron transfer rate in the conductive path.

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Acknowledgments

This work was supported by the Project of the National Science Foundation of People’s Republic of China (21275100), Shanghai Leading Academic Discipline Project (S30406) and Key Laboratory of Resource Chemistry of Ministry of Education.

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Authors and Affiliations

  1. Life and Environmental Science College, Shanghai Normal University, Shanghai, 200234, People’s Republic of China

    Peiyi Gao, Yunfeng Xia, Lele Yang, Tengfei Ma, Ling Yang, Qianqiong Guo & Shasheng Huang

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  1. Peiyi Gao
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  2. Yunfeng Xia
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  3. Lele Yang
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  4. Tengfei Ma
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  5. Ling Yang
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Correspondence to Shasheng Huang.

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Gao, P., Xia, Y., Yang, L. et al. Aptasensor for adenosine triphosphate based on electrode–supported lipid bilayer membrane. Microchim Acta 181, 205–212 (2014). https://doi.org/10.1007/s00604-013-1100-x

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  • DOI: https://doi.org/10.1007/s00604-013-1100-x

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