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Evaluation of the healing effect of honey and colostrum in treatment of cutaneous wound in rat

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Comparative Clinical Pathology Aims and scope Submit manuscript

An Erratum to this article was published on 20 January 2017

Abstract

Wound healing is a dynamic and complex process of replacing devitalized and missing cellular structures and tissue layers. Due to the importance of wound healing, this investigation was designed to compare the effect of treatment using honey and colostrum on wound-healing process in rats. In this study, 80 adult female Sprague Dawley rats were randomly divided into four groups as negative control group, groups II, III, and IV were treated by honey, bovine colostrum, and honey + colostrum, respectively. Rats were anesthetized, hair was removed from the back, and then a wound was made on the back. Visual observation, histopathological examination, and biomechanical study were performed on days 3, 7, 14, and 21 after operation. Wound area in rats which taken colostrum and honey was lower than other groups. Promotion of wound contraction and epithelialization in rats that were treated with honey and colostrum was better than the others. Biomechanical parameters in animals were treated by colostrum and honey significantly more than other rats. A combination of honey and colostrum on wounds can enhance healing better than honey and colostrum separately.

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References

  • Alizadeh AM, Sohanaki H, Khaniki M, Mohaghgheghi MA, Ghmami G, Mosavi M (2011) The effect of Teucrium Polium Hney on the wound healing and tensile strength in rat. Iran J Basic Med 14(6):499

    Google Scholar 

  • Allen K, Hutchinson G, Molan P (2000) The potential for using honey to treat wounds infected with MRSA and VRE. In: First World Wound Healing Congress, pp 10–13

  • Anyanechi C, Saheeb B (2015) Honey and wound dehiscence: a study of surgical wounds in the mandibular bed. Niger J Clin Pract 18:251–255

    Article  CAS  PubMed  Google Scholar 

  • Cheng PG, Phan CW, Sabaratnam V, Abdullah N, Abdulla MA, Kuppusamy UR (2013) Polysaccharides-rich extract of Ganoderma lucidum (MA Curtis: Fr.) P. Karst accelerates wound healing in streptozotocin-induced diabetic rats. Evid Based Complement Alternat Med

  • Cooke J, Dryden M, Patton T, Brennan J, Barrett J (2015) The antimicrobial activity of prototype modified honeys that generate reactive oxygen species (ROS) hydrogen peroxide. BMC res notes 8:20

    Article  PubMed  PubMed Central  Google Scholar 

  • Dluya T (2015) Critical review: antioxidant properties and antibiotic mechanism of honey against infectious diseases. Int J Adv Life Sci Technol 2:16–24

    Article  Google Scholar 

  • Esmaeili H, Dehghan M, Rabbani M, Haririan E (2014) Effect of bovine colostrum on open wound healing in Guinea pigs. Razi J Med Sci 21:66–74

    Google Scholar 

  • Gauthier S, Pouliot Y, Maubois J (2006) Growth factors from bovine milk and colostrum: composition, extraction and biological activities. Lait 86:99–125

    Article  CAS  Google Scholar 

  • Ghasemi S, Emami M, Maleki M, Fathi B (2012) Histhopathologic evaluation of curative impact of Aloe vera L. fresh gel on healing of experimental infected full-thickness open wounds induced with Staphylococcus aureus in dogs. Iran J Vet Surg 4:103–114

    Google Scholar 

  • Ginjala V, Pakkanen R (1998) Determination of transforming growth factor-β1 (TGF-β1) and insulin-like growth factor 1 (IGF-1) in bovine colostrum samples. J Immunoass Immunochem 19:195–207

    Article  CAS  Google Scholar 

  • Hampton S, Coulborn A, Tadej M, Bree-Aslan C (2011) Using a superabsorbent dressing and antimicrobial for a venous ulcer. Br J Nurs 20:38–S43

    Article  Google Scholar 

  • Jenkins R, Burton N, Cooper R (2013) Proteomic and genomic analysis of methicillin-resistant Staphylococcus aureus (MRSA) exposed to manuka honey in vitro demonstrated down-regulation of virulence markers. J Antimicrob Chemother:1–13

  • Jull A, Walker N, Deshpande S (2013) Honey as a topical treatment for wounds. Cochrane Database Syst Rev 2(2)

  • Kamer E, Unalp HR, Gundogan O, Diniz G, Ortac R, Olukman M, Derici H, Onal M (2010) Effect of ascorbic acid on incisional wound healing in streptozotocin-induced diabetic rats. Wounds 22(2):27–31

    PubMed  Google Scholar 

  • Kshirsagar AY, Vekariya MA, Gupta V, Pednekar AS, Mahna A, Patankar R, Shaikh A, Nagur B (2015) A comparative study of colostrum dressing versus conventional dressing in deep wounds. J Clin Diagn Res 9(4):1

    Google Scholar 

  • Kwakman P, Zaat S (2012) Antibacterial components of honey. IUBMB life 64:48–55

    Article  CAS  PubMed  Google Scholar 

  • Mahendran S, Kumarasamy D (2015) Antimicrobial activity of some honey samples against pathogenic bacteria. Int Lett Nat Sci 7:15–20

    Article  Google Scholar 

  • Mohammed H, Salama M, El Lenjawi B, Hassan I (2015) Using honey in post-excision malignant melanoma ulcers. J Lymphoedema 7:41–45

    Google Scholar 

  • Molan P (2011) The evidence and the rationale for the use of honey as wound dressing. Wound Prac Res 19:204–220

    Google Scholar 

  • Nisbet HO, Nisbet C, Yarim M, Guler A, Ozak A (2010) Effects of three types of honey on cutaneous wound healing. Wounds Compendium Clin Res Prac 22(11):275–283

    Google Scholar 

  • Noda K, Umeda M, Ono T (1984) Transforming growth Factors A and B (TGF A & B) in bovine colostrum were involved in normal cellular activities. Gann 75:109–112

    CAS  PubMed  Google Scholar 

  • Oryan A, Moshiri A (2011) A long term study on the role of exogenous human recombinant basic fibroblast growth factor on the superficial digital flexor tendon healing in rabbits. J Musculoskelet Neuronal Interact 11:185–195

    CAS  PubMed  Google Scholar 

  • Oryan A, Zaker S (1998) Effects of topical application of honey on cutaneous wound healing in rabbits. J Vet Med A 45:181–188

    Article  CAS  Google Scholar 

  • Reddy N (2013) Lantana camara Linn. chemical constituents and medicinal properties: a review. Scholars Acad J Pharmacy 2:445–448

    Google Scholar 

  • Sanjay C, Ritura J (2015) Deep wounds: colostrum dressing versus conventional dressing. Int j interdiscip multidiscip stud 2:24–28

    Google Scholar 

  • Sazegar G, Attarzadeh Hosseini SR, Behravan E (2011) The effects of supplemental zinc and honey on wound healing in rats. Iran J Basic Med Sci 14:391–398

    CAS  PubMed  PubMed Central  Google Scholar 

  • Shin JM, Jeong YJ, Cho HJ, Park KK, Chung IK, Lee IK, Kwak JY, Chang HW, Kim CH, Moon SK, Kim WJ (2013) Melittin suppresses HIF-1α/VEGF expression through inhibition of ERK and mTOR/p70S6K pathway in human cervical carcinoma cells. PLoS One 8(7):e69380

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Stelwagen K, Carpenter E, Haigh B, Hodgkinson A, Wheeler T (2009) Immune components of bovine colostrum and milk. J Anim Sci 87:3–9

    Article  CAS  PubMed  Google Scholar 

  • Stout E, McKessor A (2012) Glycerin-based hydrogel for infection control. Adv Wound Care 1:48–51

    Article  Google Scholar 

  • Takayama Y, Kitsunai K, Mizumachi K (2001) Factors in bovine colostrum that enhance the migration of human fibroblasts in type I collagen gels. Biosci Biotechnol Biochem 65(12):2776–2779

    Article  CAS  PubMed  Google Scholar 

  • Tan HT, Rahman RA, Gan SH, Halim AS, Hassan SA, Sulaiman SA, Kirnpal-Kaur BS (2009) The antibacterial properties of Malaysian tualang honey against wound and enteric microorganisms in comparison to manuka honey. BMC Complement Altern Med 9(1):34

    Article  PubMed  PubMed Central  Google Scholar 

  • Tan M, Hasan Adli D, Tumiran M, Abdulla M, Yusoff K (2012) The efficacy of Gelam honey dressing towards excisional wound healing. Evid Based Complement Alternat Med :1–6

  • Thapa B (2005) Health factors in colostrum. Indian J Pediatr 72:579–581

    Article  CAS  PubMed  Google Scholar 

  • Torre C, Jeusette I, Serra M, Brazis P, Puigdemont A (2006) Bovine colostrum increases proliferation of canine skin fibroblasts. J Nutr 136:2058–2060

    Google Scholar 

  • Vandamme L, Heyneman A, Hoeksema H, Verbelen J, Monstrey S (2013) Honey in modern wound care: a systematic review. Burns 39:1514–1525

    Article  CAS  PubMed  Google Scholar 

  • Voicu G, Oprea O, Vasile B, Andronescu E (2013) Antibacterial activity of zinc oxide–gentamicin hybrid material. Digest J Nanomater Biostruc 8:1191–1203

    Google Scholar 

  • Yaghoobi R, Kazerouni A (2013) Evidence for clinical use of honey in wound healing as an anti-bacterial, anti-inflammatory anti-oxidant and anti-viral agent: a review. Jundishapur J Nat Pharm Prod 8:100–104

    Article  PubMed  PubMed Central  Google Scholar 

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Acknowledgments

This research was financially supported by the Shiraz University of Medical Sciences, Vice-Chancellor for Research, Shiraz, Iran.

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Authors and Affiliations

  1. Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

    Nader Tanideh

  2. Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

    Nader Tanideh

  3. Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran

    Ehsan Abdordideh

  4. Student Research Committee, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

    Seyedeh Leili Asadi Yousefabad

  5. Young Researchers and Elite Club, Yasuj Branch, Islamic Azad University, Moalem St., Shahed 22 Avenue, P.O. Box: 75917-34715, Yasuj, Iran

    Sajad Daneshi

  6. Laboratory Animals Center, Shiraz University of Medical Sciences, Shiraz, Iran

    Omid Koohi Hosseinabadi

  7. Center for Nanotechnology in Drug Delivery, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran

    Soleiman Mohammadi Samani

  8. Basic Sciences in Infection Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

    Amin Derakhshan far

Authors
  1. Nader Tanideh
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  2. Ehsan Abdordideh
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  3. Seyedeh Leili Asadi Yousefabad
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  4. Sajad Daneshi
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  5. Omid Koohi Hosseinabadi
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  6. Soleiman Mohammadi Samani
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Correspondence to Sajad Daneshi.

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The authors declare that they have no conflict of interest.

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There was no funding.

Ethical approval

All applicable international, national, and institutional guidelines for the care and use of animals were followed and approved by the Animal Ethics Committee of Shiraz University of Medical Sciences, Shiraz, Iran and administered by the National Animal Ethics Advisory Committee.

Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/s00580-017-2413-7.

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Tanideh, N., Abdordideh, E., Yousefabad, S.L.A. et al. Evaluation of the healing effect of honey and colostrum in treatment of cutaneous wound in rat. Comp Clin Pathol 26, 71–77 (2017). https://doi.org/10.1007/s00580-016-2347-5

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  • DOI: https://doi.org/10.1007/s00580-016-2347-5

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