Abstract
Punica granatum is a shrub or tree, 8 to 10 m high from the Punicaceae family, and grows wild in Iran. Flowers are regular, solitary, or in fascicles at apices, with a size of 4 to 6 cm. Petals are lanceolate, wrinkled, and brilliant orange-red. Bleeding (hemorrhage) is the loss of blood or blood escape from the circulatory system. The aim of this study was to examine the effect of pomegranate flower extract on coagulation parameters in rats. Eighty-four Sprague–Dawley male rats, divided into twelve groups, each comprising seven rats, were used in the present study. Animals had free access to food but were deprived of drinking water for 24 h before warfarin injection. Hydroalcoholic extract of P. granatum flowers and tranexamic acid (various doses) were administered once daily for 7 days in warfarin-treated groups. Blood samples were obtained by inserting a needle into the heart, and their plasma was analyzed for prothrombin time (PT), partial thromboplastin time (PTT), platelet (PLT) count, fibrinogen, d-dimer, and euglobulin lysis time (ELT). The present study showed that administration of pomegranate flower extract led to a significant change in PTT, PLT count, and fibrinogen level and a significant decrease in PT level. There was no significant difference in d-dimer level between groups. In this study, animals showed no disseminated intravascular coagulation so no significant difference between control group and treatment in ELT test. It can be concluded that administration of pomegranate flower extract has negligible positive effects on the early stage of experimentally induced bleeding disorder in rats.
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References
Adam SS, Key NS, Greenberg CS (2009) D-dimer antigen: current concepts and future prospects. Blood 113:2878–2887
Ahangarpour A, Heidari R, Abdolahzadeh M, Oroojan AA (2012) Antispasmodic effects of aqueous and hydroalcoholic Punica granatum flower extracts on the uterus of non-pregnant rats. J Reprod Infertil 13:30–34
Asgary S, Najafi S, Ghannadi A, Dashti G, Helalat A (2012) Efficiency of Black cumin seeds on hematological factors in normal and hypercholesterolemic rabbits. ARYA Atheroscler 7:146–150
Attilio P, Merritt C, Sims J, Kane N, Osullivan J (2010) The effect of ellagic acid on platelet activation as PLT as measured by the quantification of p-selective using slow cytometry. AANA J 786:453–459
Cliffton EE, Girolami A, Agostino D (2013) The effect of ellagic acid on the thrombin-induced hemorrhagic syndrome in the rat. Blood J 9:253–256
Deonarine P, Wet CD, McGhee A (2012) Computed tomographic pulmonary angiography and pulmonary embolism: predictive value of a D-dimer assay. BMC Res Notes 5:104
Girolami A, Agostino D, Cliffton EE (2013) The effect of ellagic acid on coagulation. Blood J 9:93–102
Jayashankar S, Panagoda GJ, Amaratunga EA, Perera K, Rajapakse PS (2011) A randomized double-blind placebo-controlled study on the effects of a herbal toothpaste on gingival bleeding, oral hygiene and microbial variables. Ceylon 56:5–9
Jurenka J (2008) Therapeutic applications of Pomegranate (Punica granatum L.): a review. Alternat Med Rev 13:128–144
Kaur G, Jabbar Z, Athar M, Sarwar Alam M (2006) Punica granatum (pomegranate) flower extract possesses potent antioxidant activity and abrogates Fe-NTA induced hepatotoxicity in mice. Food Chem Toxicol 44:984–993
Lijnen HR, Collen D (2008) Molecular and cellular basis of fibrinolysis. In: Hoffman R, Benz EJ Jr, Shattil SJ (eds) Hoffman hematology: basic principles and practice, 5th edn. Churchill Livingstone Elsevier, Philadelphia, Pa, Chap 119
Longo DL, Fauci AS, Kasper DL, Hauser SL, Jameson JL, Loscalzo J (2012) Harrison's principles of internal medicine, 18th edn. McGraw-Hill, New York
Paez X, Hernandez L (2003) Topical hemostatic effect of a common ornamental plant, the Geraniaceae Pelargonium zonale. J Clin Pharmacol 43:291–295
Schouten HJ, Geersing GJ, Koek HL, Zuithoff NPA, Janssen KJM, Douma RA, van Delden JJM, Moons KGM, Reitsma JB (2013) Diagnostic accuracy of conventional or age adjusted D-dimmer cut-off values in older patients with suspected venous thromboembolism: systematic review and meta-analysis. Bri Med J 346:2492
Sina B (1388) Low in medicine. Punica granatum Linn. flower. Tehran, Shahid Beheshte Medical University
Tangade PS, Mathur A, Tirth A, Kabasi S (2012) Anti-gingivitis effects of Acacia arabica-containing toothpaste. China J Dent Res 15:49–53
Wells PS, Anderson DR, Rodger M (2003) Evaluation of D-dimer in the diagnosis of suspected deep-vein thrombosis. N Engl J Med 349(13):1227–1235
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The authors would like to thank the Research Council of Shiraz University and School of Veterinary Medicine, Shiraz University, for the financial and technical support of this study.
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Safavi, K., Rajaian, H. & Nazifi, S. The effect of hydroalcoholic extract of Punica granatum flower on coagulation parameters in rats. Comp Clin Pathol 23, 1757–1762 (2014). https://doi.org/10.1007/s00580-014-1993-8
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DOI: https://doi.org/10.1007/s00580-014-1993-8