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Collagen metabolism and antioxidant status in the paw tissue of type II collagen-induced arthritic rats—protective role of alginic acid isolated from Sargassum wightii

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Abstract

The aim of the present study was to evaluate the effects of alginic acid isolated from the brown algae, Sargassum wightii, on collagen metabolism and antioxidant status in the paw tissue of rats in experimental arthritis. To induce arthritis, rats were immunized with type II collagen (2 mg/ml) intradermally. Alginic acid (100 mg/kg) and indomethacin (3 mg/kg) were administered orally for 21 days. The severity of arthritis was assessed by measuring paw edema volume and histology of hind paw tissue. Markers of oxidative stress, protein-bound carbohydrates in plasma, and urine and degradative collagen levels were analyzed in all the rats. Alginic acid treatment significantly reduced the oxidative stress in the paw tissue of arthritic rats and modulated the altered level of collagen metabolism along with reduction in serum inducible nitric oxide synthase (iNOS) activity, prostaglandins E2 level, and paw edema volume in arthritic rats. Histopathology of hind paw tissue showed that arthritic rats receiving alginic acid exhibited the dermis and hypodermis free from inflammatory cell infiltration with mild edema and proliferation of collageneous tissue. The overall results show that alginic acid offers protection against arthritis via reducing oxidative stress, modulating collagen metabolism, and downregulating the activities of iNOS and prostaglandin E2.

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References

  • Benke GM, Cheever KC (1974) Comparative toxicity, anticholinesterase action and metabolism of methyl parathion and parathion in sunfish and mice. Toxicol Appl 28(1):97–109

    Article  CAS  Google Scholar 

  • Bitter T, Muir HM (1962) Modified uronic acid carbazole reaction. Ann Biochem 4:330–334

    Article  CAS  Google Scholar 

  • Boas NF (1953) Method for the determination of hexosamines in tissues. J Biol Chem 204:553–563

  • Buchanan MJ, Isdale IC, Rose BS (1965) Uric acid estimation chemical and enzymatic methods compared. Ann Rheum Dis 25(2):184–185

    Google Scholar 

  • Budhiyanti SA, Raharjo S, Djagal W (2011) Free radical scavenging, metal chelating and singlet oxygen quenching activity of fractionated brown seaweed Sargassum hystrix extract. J Biol Sci 11:288–298

    Article  CAS  Google Scholar 

  • Cronstein BN, Terkeltaub R (2006) The inflammatory process of gout and its treatment. Arthritis Res Ther 8(1):S3. doi:10.1186/ar1908

    Article  PubMed Central  PubMed  Google Scholar 

  • Daoussis D, Kitas DG (2010) Uric acid and cardiovascular risk in rheumatoid arthritis. Rheumatology 50:1354–1355

    Article  PubMed  Google Scholar 

  • David M, Richard JS (1983) Glutathione reductase. In: Bermeyer, Hans, Ulrich, Jr. (Eds.). Methods of enzymatic analysis. 258–265

  • Dwivedi S, Singh S, Jaiswa G (2014) Lipid-peroxidation & non-enzymatic antioxidant (vitamin e & vitamin c) serum levels in anemic rheumatoid arthritis patients. J Pharm Biol Sci 9:01–03

  • Fenoradosoa TA, Ali G, Delattre C, Celine Laroche C (2010) Extraction and characterization of an alginate from the brown seaweed Sargassum turbinarioides Grunow. J Appl Phycol 22:131–137

    Article  CAS  Google Scholar 

  • Gatea F, Petrareanu C, Lupeanu E, Radu GL (2014) Plasma thiol concentration levels and their correlation with health patients condition. U.P.B. Sci. Bull Vol 76:1454–2331

    Google Scholar 

  • Grasdalen H (1983) High field 1-H NMR spectroscopy of alginates: sequential structure and linkage conformation. Carbohydr Res 118:255–260

  • Hu ML (1994) Measurement of protein thiol groups and glutathione in plasma. Methods Enzymol 233:380–385

    Article  CAS  PubMed  Google Scholar 

  • Kakkar P, Das B, Viswanathanan PN (1984) A modified spectrophotometric assay of SOD. Ind J Biochem Biophys 21:130–132

    CAS  Google Scholar 

  • Khandhadiya PK, Chianeh YR, Pragna R (2014) Role of serum copper and ceruloplasmin level in patients with dysfunctional uterine bleeding. Int J Reprod Contracept Obstet Gynecol 3(2):333–334

    Article  Google Scholar 

  • Kumar V, Gupta M, Jethani S (2013) Effect of indomethacin on colon of albino rat histopathological study. Int J Biomed Adv Res 4:523–527

    Article  Google Scholar 

  • Lawrence RA, Burk RE (1976) Glutathione peroxidase activity in selenium-deficientrat liver. Biochem Biophys Res 17:952

  • Lee E G, Lee S, Chae HJ, Park SJ, Lee YC, Yoo W (2010) Ethyl acetate fraction from Cudrania tricuspidata inhibts IL-1b-induced rheumatoid synovial fibroblast proliferation and MMPs, COX-2 and PGE2 production. Biological Research 43:225–231

  • Lowry OH, Rosebrough NJ, Farr AJ, Randal RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–273

    CAS  PubMed  Google Scholar 

  • Maehly AC, Chance B (1954) The assay of catalase and peroxidases. Methods Biochem Anal 1:357–408

    Article  CAS  PubMed  Google Scholar 

  • Neuman RE, Logan MA (1950) The determination of collagen and elastin in tissues. J Biol Chem 186:549–556

    CAS  PubMed  Google Scholar 

  • Ohkawa H, Ohishi H, Yagi K (1979) Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal Biochem 95:351–358

    Article  CAS  PubMed  Google Scholar 

  • Osredkar J, Sustar N (2011) Copper and zinc biological role and significance of copper/zincimbalance. J Clin Toxicol S3:001

  • Parihar G, Sharma A, Ghule S, Sharma P, Deshmukh P, Srivastava DN (2011)Anti inflammatory effect of Calotropis procera root bark extract. Asian J Pharm Life Sci 1:29–44

    Google Scholar 

  • Pathak NL, Patel DD, Patel BA, Bhattayana M, Patel PD (2011) Free radical scavenging activity of Mangifera indica methanolic extract in arthritic rats. Int Res J Pharm 2(6):148–152

    Google Scholar 

  • Petchi RR, Vijaya C (2012) Anti-diabetic and anti arthritic potential of Glycosmis pentaphylla stem bark in FCA induced arthritis and streptozotocin induced diabetic rats. Pharmacol Int J Pharma Bio Sci 3(3):328–336

  • Prathapan A, Cherian OL, Nampoothiri SV, Mini S, Raghu KG (2011) In vitro antiperoxidate, free radical scavenging and xanthine oxidase inhibitory potential of ethyl acetate fraction of Saraca ashoka flowers. Nat Prod Res 25(3):298–309

    Article  CAS  PubMed  Google Scholar 

  • Salter M, Knowles RG (1997) Assay of NOS activity by the measurements of conversion of oxy hemoglobin to methemoglobin by NO. In: Titheradge MA (ed)Methods in molecular biology nitric oxide, vol 100. Humana press, Protocol TOTOWA, N.J, pp 61–65

    Google Scholar 

  • Saravanan S, Babu NP, Pandikumar P, Ignacimuthu S (2011) Therapeutic effect of Saraca asoca (Roxb.) Wilde on lysosomal enzymes and collagen metabolism in adjuvant induced arthrtis. Inflammopharmacology 19:317–325

  • Sarithakumari CH, Kurup GM (2013) Alginic acid isolated from Sargassum wightii exhibits anti-inflammatory potential on type II collagen induced arthritis in experimental animals. Int Immunopharmacol 17:1108–1115

  • Sarithakumari CH, Renju GL, Kurup GM (2013) Anti-inflammatory and antioxidant potential of alginic acid isolated from the marine algae, Sargassum wightii on adjuvant-induced arthritic rats. Inflamm Pharamacol 21:261–268

  • Sathyavathi A, Paramesha S, Suchetha Kumari N, Damodara Gowda KM (2011) Antioxidant status and serum total protein levels in elderly women. Int J Appl Biol Pharm Technol 2:521–524

    Google Scholar 

  • Shobharani P, Nanishankar VH, Halami PM, Sachindra NM (2014) Antioxidant and anticoagulant activity of polyphenol and polysaccharides from fermented Sargassum sp. Int J Biol Macromol 65:542–548

    Article  CAS  PubMed  Google Scholar 

  • Subramoniam A, Madhavachandran V, Gangaprasad A (2013) Medicinal plants in the treatment of arthritis. Ann Phytomedicine 2:3–36

    Google Scholar 

  • Umar S, Asif M, Sajad M, Ansari MM (2012) Anti-inflammatoryand antioxidant activity of Trachyspermum ammi seeds in collagen induced arthritis in rats. Int J Drug Dev Res 4(1):210–219

  • Wagner WD (1959) More sensitive assay discriminating galactosamine and glucosamine in mixtures. Ann Biochem 94:394–396

    Article  Google Scholar 

  • Warren L (1959) The thiobarbituric acid assay of sialic acid. J Biol Chem 234:1971–1975

    CAS  PubMed  Google Scholar 

  • Whiteman P (1973) The quantitative measurement of Alician Blue-glycoaminoglycan complexes. Biochem J 131:343–350

  • Woessner JF (1961) The determination of hydroxyproline line in tissue and protein samples containing small portions of this amino acid. Arch Biochem Biophys 93:440–447

    Article  CAS  PubMed  Google Scholar 

  • Zahidah AF, Faizah O, Nur Aqilah K, Taty Anna K (2012) Curcumin as an anti-arthritic agent in collagen-induced arthritic sprague-dawley rats. Sains Malaysiana 41(5):591–595

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Acknowledgments

Financial assistance from UGC in the form of RGNF is gratefully acknowledged. We express our sincere gratitude to Mr. Jayaram V (cytotechnologist, Med Care Diagnostic Laboratory, Parippally, Kollam, Kerala) for helping us with the histopathological studies.

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  1. Department of Biochemistry, University of Kerala, Kariavattom Campus, Trivandrum, Kerala, 695581, India

    C. H. Sarithakumari & G. Muraleedhara Kurup

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  1. C. H. Sarithakumari
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  2. G. Muraleedhara Kurup
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Correspondence to G. Muraleedhara Kurup.

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Sarithakumari, C.H., Kurup, G.M. Collagen metabolism and antioxidant status in the paw tissue of type II collagen-induced arthritic rats—protective role of alginic acid isolated from Sargassum wightii . Comp Clin Pathol 24, 669–676 (2015). https://doi.org/10.1007/s00580-014-1965-z

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  • DOI: https://doi.org/10.1007/s00580-014-1965-z

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