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Lipoproteins profile, acute phase proteins, proinflammatory cytokines and oxidative stress biomarkers in sheep with pneumonic pasteurellosis

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Abstract

The aim of this study was to assess the pathophysiological importance of lipid profile, acute phase proteins, proinflammatory cytokines and oxidative stress markers in sheep with pneumonic pasteurellosis. Blood samples were collected from 36 Pasteurella multocida-infected sheep, together with 20 healthy controls. Samples for bacteriological examination (nasal swabs, bronchoalveolar lavage) were collected from all animals and subjected to bacteriological examinations. Moreover, heart blood and lung samples were collected from the dead pneumonic sheep and subjected also to bacteriological examinations. A lipid profile was determined, along with a blood picture and other biochemical parameters. The acute phase proteins (fibrinogen, haptoglobin, serum amyloid A), the proinflammatory cytokine tumour necrosis factor-alpha, interleukins (IL-1α, IL-1β, IL-6), interferon-gamma and the oxidative stress markers malondialdehyde, super oxide dismutase, glutathione and catalase were also measured. The examined biochemical parameters were increased in the pneumonic sheep, except for cholesterol and high-density lipoprotein cholesterol (HDL-c), which were significantly lower than control group. Acute phase proteins and cytokines were significantly higher in the pneumonic sheep when compared to the healthy sheep. There was a significant increase in the levels of malondialdehyde; however, a significant decrease in the levels of super oxide dismutase, glutathione and catalase was observed. The present study shed the light on the possible pathphysiological role of lipid profile, acute phase proteins (APPs), proinflammatory cytokines and oxidative stress markers in pneumonic pasteurelosis in sheep.

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Acknowledgments

The authors would like to thank N. Peachy, English professor, Deanship for Educational Services, Qassim University, for language revising. The authors would like also to thank Deanship of Scientific Research, King Faisal University for the financial support of the research (project No. 130031).

Conflict of interest

There are no conflicts of interests professionally or financially with this manuscript that the co-authors are aware of.

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  1. Mohamed Tharwat

    Present address: Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt

Authors and Affiliations

  1. Department of Clinical Studies, College of Veterinary Medicine and Animal Resources, King Faisal University, P.O. Box 400, Al-Ahsa, 31982, Saudi Arabia

    Wael M. El-Deeb

  2. Department of Internal Medicine, Infectious Diseases and Fish Diseases, Faculty of Veterinary Medicine, Mansoura University, EL-Gomhorya St, Mansoura, Dakahlia, 35516, Egypt

    Wael M. El-Deeb

  3. Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Qassim, Saudi Arabia

    Mohamed Tharwat

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El-Deeb, W.M., Tharwat, M. Lipoproteins profile, acute phase proteins, proinflammatory cytokines and oxidative stress biomarkers in sheep with pneumonic pasteurellosis. Comp Clin Pathol 24, 581–588 (2015). https://doi.org/10.1007/s00580-014-1949-z

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