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Effects of pentoxifylline on skeletal muscle injury induced by acute hindlimb ischaemia–reperfusion: a histopathological study in a rat model

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Abstract

Skeletal muscle ischaemia–reperfusion injury can occur with diseases, trauma and during orthopaedic surgical procedures. Ischaemia–reperfusion injury is characterized by the production of oxygen free radicals leading to disturbances in vasomotility and microvascular permeability. This study evaluated the effects of pentoxifylline, a methylxanthine derivative, on skeletal muscle injury after acute hindlimb ischaemia–reperfusion. Twenty male Wistar rats were allocated randomly into two groups: ischaemia–reperfusion and ischaemia–reperfusion + pentoxifylline. All animals underwent 2 h of ischaemia by occlusion of the femoral artery followed by 24 h of reperfusion. Rats in ischaemia–reperfusion + pentoxifylline group were given a dose of intraperitoneal pentoxifylline injection 40 mg/kg immediately before reperfusion. After the reperfusion period, animals were euthanized and left gastrocnemius muscle harvested for histopathological analysis under light microscopy. In the ischaemia–reperfusion group, the changes were more intense with fibre disruption, neutrophil infiltration and interstitial oedema. There was a significant difference in terms of pathological parameters between ischaemia–reperfusion + pentoxifylline and ischaemia–reperfusion groups as histopathological scores (P < 0.05). The administration of pentoxifylline significantly decreased skeletal muscle injury induced by acute hindlimb ischaemia–reperfusion according to our histological findings.

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Authors and Affiliations

  1. College of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran

    Hamed Ashrafzadeh Takhtfooladi

  2. Department of Pathobiology, College of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran

    Fariborz Moayer

  3. Department of Surgery, College of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran

    Mohammad Abarkar

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  1. Hamed Ashrafzadeh Takhtfooladi
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  2. Fariborz Moayer
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  3. Mohammad Abarkar
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Correspondence to Fariborz Moayer.

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Takhtfooladi, H.A., Moayer, F. & Abarkar, M. Effects of pentoxifylline on skeletal muscle injury induced by acute hindlimb ischaemia–reperfusion: a histopathological study in a rat model. Comp Clin Pathol 24, 545–548 (2015). https://doi.org/10.1007/s00580-014-1941-7

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  • DOI: https://doi.org/10.1007/s00580-014-1941-7

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