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Free radical quenching effect of Shemamruthaa—a Siddha drug in 7,12-dimethylbenz(a)anthracene-induced experimental breast cancer rats

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Abstract

This study aims to evaluate the modulatory effect of Shemamruthaa (SM), a modified indigenous Siddha formulation, on the hematological indices, membrane-bound enzymes, enzymatic antioxidants free radical levels in cancer rats and also on the histologicl alterations in the mammary tissue. Mammary cancer was induced by intragastric administration of a single dose of 25 mg/kg body weight of 7,12-dimethylbenz(a)anthracene (DMBA), and cancer-induced rats were treated with oral administration of SM at a dosage of 400 mg/kg body weight dissolved in water for 14 days. Hematological indices such as hemoglobin (HB), red blood cells (RBC), white blood cells (WBC), platelets counts and packed cell volume (PCV) were decreased, whereas erythrocyte sedimentation rate (ESR) was increased. Moreover, lipid peroxidation and free radicals were found to be increased, whereas activities of antioxidant enzymes and membrane-bound ATPases were decreased in cancer-bearing animals when compared with control rats. The drug SM also effectively restored morphological alterations in the mammary tissue of cancer-bearing rats. From the results of the present study, the beneficial effect of SM on DMBA-induced mammary carcinoma is evident.

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  1. Department of Medical Biochemistry, Dr. ALM Post-Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, India

    Nandhakumar Elumalai, Purushothaman Ayyakkannu & Sachdanandam Panchanadham

  2. Department of Pathology, Dr. ALM Post-Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, 600113, India

    Shanthi palanivelu

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  1. Nandhakumar Elumalai
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Elumalai, N., Ayyakkannu, P., palanivelu, S. et al. Free radical quenching effect of Shemamruthaa—a Siddha drug in 7,12-dimethylbenz(a)anthracene-induced experimental breast cancer rats. Comp Clin Pathol 23, 1703–1713 (2014). https://doi.org/10.1007/s00580-014-1914-x

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