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A field outbreak of aflatoxicosis with high fatality rate in feedlot calves in Iran

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Abstract

An outbreak of aflatoxicosis is reported in calves in Iran. Aflatoxicosis is caused by aflatoxins and is the most important disease resulting from ingestion of feed contaminated with Aspergillus spp. Two hundred 4- to 10-month-old male Holstein calves of approximately 150 kg were kept in a farm and were fed a ration consisting of alfalfa hay and sunflower cakes. A total of 35 calves (17%) died 4 months after presenting a disease characterised by general unthriftiness, diarrhoea and death. Necropsy findings included firm, pale livers and hydrothorax. Lymphopenia, monocytosis, high levels of serum sorbitol dehydrogenase (SDH), alkaline phosphatase (ALP) and SGOT in the serum and congestion, necrosis, fatty change and megalocytosis in the necropsied livers were found on laboratory examination. Reverse transcription-polymerase chain reaction detected bovine viral diarrhoea (BVD) virus genome in the affected calves. Toxicogenic species of Aspergillus (mainly A. flavus) grew in the culture from sunflower feeds. The analysis of the feed revealed 1,130 μg of AFB1/kg. A diagnosis of aflatoxicosis due to the immunosuppressive effects of the BVD-MD infection was made based on the characteristic clinical signs, pathology, virology, haematology, biochemistry and mycology, and on the presence of high levels of aflatoxin B1 in the sunflower fed to the calves. Providing aflatoxin-free feeds to calves prevented further clinical cases. This report demonstrates the need for careful quality control of feed products and the susceptibility of immunosuppressive calves to the effects of aflatoxins.

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Acknowledgements

The authors thank the research vice counselor of the University of Tabriz for the financial support and Mrs. M. Hashemian, Mycological Department, University of Tehran, for her assistance.

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The authors declare no conflicts of interest.

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Authors and Affiliations

  1. Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, 29 Bahman Boulvar, Tabriz, Iran

    Mohamad Tolouei Kaleibar

  2. Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

    Javad Ashrafi Helan

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  1. Mohamad Tolouei Kaleibar
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  2. Javad Ashrafi Helan
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Correspondence to Mohamad Tolouei Kaleibar.

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support for the research performed in the of Faculty of Veterinary Medicine, University of Tabriz

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Kaleibar, M.T., Helan, J.A. A field outbreak of aflatoxicosis with high fatality rate in feedlot calves in Iran. Comp Clin Pathol 22, 1155–1163 (2013). https://doi.org/10.1007/s00580-012-1543-1

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  • DOI: https://doi.org/10.1007/s00580-012-1543-1

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