Abstract
Nanoparticles, such as colloids, liposomes, and extracellular vesicles, have important roles in a wide range of processes and applications. The detection and characterization of nanoparticles remain technological hurdles because of the small size of nanoparticles, especially when they are heterogeneous. Resistive-pulse sensing technology measures nanoparticles on a single-particle basis. We investigate multiple parameters, including shape and size of the nanoparticles and pores, affecting the transmembrane, blockade of ion current, or the resistive pulse. Finite-element simulations and experimental results agreed reasonable well with each other when monodisperse carboxylated polystyrene nanospheres were used. Compared to using a cylindrical pore, resistive pulses of larger magnitudes were generated using a conical pore of the same diameter. In addition, analyses of the resistive pulse provided information on the shape of nanoparticles, such as spheres or cylinders. This methodology, we believe, open opportunities to improve the characterization of nanoparticles.
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References
Branton D et al (2008) The potential and challenges of nanopore sequencing. Nat Biotechnol 26:1146–1153. doi:10.1038/Nbt.1495
Deblois RW, Bean CP (1970) Counting and sizing of submicron particles by resistive pulse technique. Rev Sci Instrum 41:909. doi:10.1063/1.1684724
Deblois RW, Bean CP, Wesley RKA (1977) Electrokinetic measurements with submicron particles and pores by resistive pulse technique. J Colloid Interface Sci 61:323–335. doi:10.1016/0021-9797(77)90395-2
Dekker C (2007) Solid-state nanopores. Nat Nanotechnol 2:209–215. doi:10.1038/nnano.2007.27
Fraikin J-L, Teesalu T, McKenney CM, Ruoslahti E, Cleland AN (2011) A high-throughput label-free nanoparticle analyser. Nat Nanotechnol 6:308–313. doi:10.1038/nnano.2011.24
Gardiner C, Ferreira YJ, Dragovic RA, Redman CW, Sargent IL (2013) Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis. J Extracell Vesicles 2:19671
Garza-Licudine E, Deo D, Yu S, Uz-Zaman A, Dunbar WB (2010) Portable nanoparticle quantization using a resizable nanopore instrument—the Izon qNano(™). Conf Proc IEEE Eng Med Biol Soc 2010:5736–5739. doi:10.1109/IEMBS.2010.5627861
Ito T, Sun L, Bevan MA, Crooks RM (2004) Comparison of nanoparticle size and electrophoretic mobility measurements using a carbon-nanotube-based coulter counter, dynamic light scattering, transmission electron microscopy, and phase analysis light scattering. Langmuir 20:6940–6945. doi:10.1021/la049524t
Kozak D, Anderson W, Vogel R, Trau M (2011) Advances in resistive pulse sensors: devices bridging the void between molecular and microscopic detection. Nano Today 6:531–545. doi:10.1016/j.nantod.2011.08.012
Kozak D, Anderson W, Vogel R, Chen S, Antaw F, Trau M (2012) Simultaneous sise and zeta-potential measurements of individual nanoparticles in dispersion using size-tunable pore sensors. ACS Nano 6:6990–6997. doi:10.1021/nn3020322
Lee CY, Hsiao YH, Yu JC, Hsu CW, Hsu CH, Chen C (2014) Measurement and modeling of M13 bacteriophages transport in conical-shaped nanopores. ECS Trans 64:51–56
Li Y-Q, Zheng Y-B, Zare RN (2012) Electrical, optical, and docking properties of conical nanopores. ACS Nano 6:993–997. doi:10.1021/nn300356d
Menestrina J, Yang C, Schiel M, Vlassiouk I, Siwy ZS (2014) Charged particles modulate local ionic concentrations and cause formation of positive peaks in resistive-pulse-based detection. J Phys Chem C 118:2391–2398. doi:10.1021/jp412135v
Roberts GS, Kozak D, Anderson W, Broom MF, Vogel R, Trau M (2010) Tunable nano/micropores for particle detection and discrimination: scanning ion occlusion spectroscopy. Small 6:2653–2658. doi:10.1002/smll.201001129
Roberts GS et al (2012) Tunable pores for measuring concentrations of synthetic and biological nanoparticle dispersions. Biosens Bioelectron 31:17–25. doi:10.1016/j.bios.2011.09.040
Sexton LT, Horne LP, Martin CR (2007) Developing synthetic conical nanopores for biosensing applications. Mol BioSyst 3:667–685. doi:10.1039/b708725j
van der Pol E et al (2014) Particle size distribution of exosomes and microvesicles determined by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing. J Thromb Haemost 12:1182–1192. doi:10.1111/jth.12602
Varga Z, Yuana Y, Grootemaat AE, van der Pol E, Gollwitzer C, Krumrey M, Nieuwland R (2014) Towards traceable size determination of extracellular vesicles. J Extracell Vesicles 3:23298
Vogel R et al (2011) Quantitative sizing of nano/microparticles with a tunable elastomeric pore sensor. Anal Chem 83:3499–3506. doi:10.1021/Ac200195n
Willmott GR et al (2010) Use of tunable nanopore blockade rates to investigate colloidal dispersions. J Phys Condens Matter. doi:10.1088/0953-8984/22/45/454116
Zhang H, Chon CH, Pan X, Li D (2009) Methods for counting particles in microfluidic applications. Microfluid Nanofluidics 7:739–749. doi:10.1007/s10404-009-0493-7
Acknowledgments
This work was supported in part by the Taiwan National Science Council grants-NSC 99-2320-B-007–005-MY2 (CC) and 101-2221-E-007-101-MY3 (CC).
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Lee, CY., Chen, C. Characterizations of nanospheres and nanorods using resistive-pulse sensing. Microsyst Technol 23, 299–304 (2017). https://doi.org/10.1007/s00542-015-2481-z
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DOI: https://doi.org/10.1007/s00542-015-2481-z