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Expression dynamics of HSP70 during chronic heat stress in Tharparkar cattle

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Abstract

Six male Tharparkar cattle aged 2–3 years were selected for the study. The animals were acclimatized in the psychrometric chamber at thermoneutral zone (TNZ) for 15 days and then exposed to 42 °C temperature up to 23 days followed by 12 days of recovery period. Physiological responses were estimated, and peripheral blood mononuclear cells (PBMCs) were isolated at TNZ on day 1, day 5, and day 12; after 6 h of heat stress exposure on day 16 to day 20, day 25, day 30, day 32, day 34, day 36, and day 38; and a recovery period on day 45 and day 50. The PBMCs were cultured to study the effect of thermal challenge on HSP70 messenger RNA (mRNA) expression pattern at different temperature-time combinations. The mRNA and protein expression of HSP70 in PBMCs along with serum extracellular HSP70 (eHSP70) was increased (P < 0.05) and showed two peaks on day 17 and day 32 (2nd and 17th days of thermal challenge, respectively). The HSP70 mRNA expression was increased (P < 0.05) in a temperature- and time-dependent manner in heat stress challenge treatment as compared to control in cultured PBMCs. HSP70 expression was found to be higher (P < 0.05) after 10 days of heat exposure (corresponds to chronic heat stress) as compared to the first 5 days of heat stress (corresponds to short-term heat stress) and control period at TNZ. The present findings indicate that HSP70 is possibly involved in heat stress adaptive response in Tharparkar cattle and the biphasic expression pattern may be providing a second window of protection during chronic heat stress.

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Acknowledgements

We thank the National Initiative on Climate Resilient Agriculture (NICRA) and the Director from the Indian Veterinary Research Institute, Izzatnagar, India, for funding this work.

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Authors and Affiliations

  1. Physiology and Climatology, Indian Veterinary Research Institute, Izzatnagar, Bareilly, UP, 243122, India

    Jaya Bharati, S.S. Dangi, V.S. Chouhan, S.R. Mishra, M.K. Bharti, V. Verma, O. Shankar, V.P. Yadav, K. Das, A. Paul, S. Bag, V.P. Maurya, G. Singh, P. Kumar & M. Sarkar

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  1. Jaya Bharati
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  2. S.S. Dangi
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  3. V.S. Chouhan
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  4. S.R. Mishra
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  5. M.K. Bharti
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  6. V. Verma
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  7. O. Shankar
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  8. V.P. Yadav
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  9. K. Das
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  10. A. Paul
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  11. S. Bag
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  12. V.P. Maurya
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  13. G. Singh
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  14. P. Kumar
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  15. M. Sarkar
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Corresponding author

Correspondence to M. Sarkar.

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All the animal experiments had prior approval from the animal ethics committee of the Indian Veterinary Research Institute (IVRI), Izzatnagar, UP, India

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The authors declare that they have no conflict of interest.

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Bharati, J., Dangi, S., Chouhan, V. et al. Expression dynamics of HSP70 during chronic heat stress in Tharparkar cattle. Int J Biometeorol 61, 1017–1027 (2017). https://doi.org/10.1007/s00484-016-1281-1

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  • DOI: https://doi.org/10.1007/s00484-016-1281-1

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