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Kinetic characteristics and modelling of growth and substrate removal by Alcaligenes faecalis strain NR

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Abstract

Alcaligenes faecalis strain NR has the capability of simultaneous ammonium and organic carbon removal under sole aerobic conditions. The growth and substrate removal characteristics of A. faecalis strain NR were studied and appropriate kinetic models were developed. The maximum substrate removal rate of NH4 +-N and TOC were determined as 2.27 mg NH4 +-N/L/h and 30.00 mg TOC/L/h, respectively with initial NH4 +-N = 80 mg/L and TOC = 800 mg/L. Single-substrate models and double-substrate models based on Monod, Contois, Moser and Teissier were employed to describe the bioprocess kinetic coefficients. As a result, two double-substrate models, Teissier-Contois and Contois-Contois, were considered to be appropriate to model growth kinetics with both NH4 +-N and TOC as limiting substrates. The kinetic constants of maximum growth rate (μ max) and half-saturation constant (K S and B S) were obtained by solving multiple equations with regression. This work can be used to further understand and predict the performance of heterotrophic nitrifiers, and thus provides specific guidance of these functional strains in practical wastewater treatment process.

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Abbreviations

a :

Constant in Logistic equation

b :

Constant in Logistic equation

k :

Constant in Logistic equation

μ :

Specific growth rate (h−1)

μ max :

Maximum specific growth rate (h−1)

S i :

Concentration of substrate (mg/L)

S N :

Concentration of NH4 +-N (mg/L)

S C :

Concentration of TOC (mg/L)

K S :

Half-saturation constant for substrate (mg/L)

K N :

Half-saturation constant for NH4 +-N (mg/L)

K C :

Half-saturation constant for TOC (mg/L)

B S :

Half-saturation constant in Contois model for substrate (g substrate/g biomass)

B N :

Half-saturation constant in Contois model for NH4 +-N (g NH4 +-N/g biomass)

B C :

Half-saturation constant in Contois model for TOC (g TOC/g biomass)

λ :

Constant in Moser model

q s :

Specific substrate removal rate (g substrate/g biomass/h)

q N :

Specific NH4 +-N removal rate (g NH4 +-N/g biomass/h)

q C :

Specific TOC removal rate (g TOC/g biomass/h)

Y G :

Maximum biomass yield coefficient (g biomass/g substrate)

Y G/N :

Maximum biomass yield coefficient for NH4 +-N (g biomass/g NH4 +-N)

Y G/C :

Maximum biomass yield coefficient for TOC (g biomass/g TOC)

m S :

Maintenance coefficient (g substrate/g biomass/h)

m N :

Maintenance coefficient for NH4 +-N (g NH4 +-N/g biomass/h)

m C :

Maintenance coefficient for TOC (g TOC/g biomass/h)

r S :

Substrate removal rate (mg substrate/L/h)

r N :

NH4 +-N removal rate (mg NH4 +-N/L/h)

r C :

TOC removal rate (mg TOC/L/h)

x :

Biomass concentration (mg/L)

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Acknowledgments

The work was supported by the National Natural Science Foundation of China (No. 51208534) and Fundamental Research Funds for the Central Universities (Project No. 106112013 CDJZR 210003). We would like also to thank National Centre for International Research of Low-carbon and Green Buildings and 111 project (No. B13041) for providing their support.

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Authors and Affiliations

  1. The Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Chongqing University, Chongqing, 400045, People’s Republic of China

    Bin Zhao & Qiang An

  2. College of Urban Construction and Environmental Engineering, Chongqing University, Chongqing, 400045, People’s Republic of China

    Jie Chen, Xia Wang & Yi Xin Zhang

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  1. Jie Chen
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  2. Bin Zhao
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Correspondence to Bin Zhao.

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Chen, J., Zhao, B., An, Q. et al. Kinetic characteristics and modelling of growth and substrate removal by Alcaligenes faecalis strain NR. Bioprocess Biosyst Eng 39, 593–601 (2016). https://doi.org/10.1007/s00449-016-1541-9

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  • DOI: https://doi.org/10.1007/s00449-016-1541-9

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