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Effects of molten-salt/ionic-liquid mixture on extraction of docosahexaenoic acid (DHA)-rich lipids from Aurantiochytrium sp. KRS101

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Abstract

In this study, lipid extraction from Aurantiochytrium sp. was performed using a molten-salt/ionic-liquid mixture. The total fatty acid content of Aurantiochytrium sp. was 478.8 mg/g cell, from which 145 mg/g cell (30.3 % of total fatty acids) of docosahexaenoic acid (DHA) was obtained. FeCl3·6H2O showed a high lipid extraction yield (207.9 mg/g cell), when compared with that of [Emim]OAc, which was only 118.1 mg/g cell; notably however, when FeCl3·6H2O was mixed with [Emim]OAc (5:1, w/w), the yield was increased to 478.6 mg/g cell. When lipid was extracted by the FeCl3·6H2O/[Emim]OAc mixture at a 5:1 (w/w) blending ratio under 90 °C, 30 min reaction conditions, the fatty acid content of the extracted lipid was a high purity 997.7 mg/g lipid, with most of the DHA having been extracted (30.2 % of total fatty acids). Overall, lipid extraction from Aurantiochytrium sp. was enhanced by the synergistic effects of the molten-salt/ionic-liquid mixture with different ions.

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Abbreviations

PUFA:

Polyunsaturated fatty acids

DHA:

Docosahexaenoic acid

[Emim]OAc:

1-ethyl-3-methyl imidazolium acetate

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Acknowledgments

This work was supported by the New & Renewable Energy Technology Development Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korea government Ministry of Knowledge Economy (No. 20123010090010) and by the Advanced Biomass R&D Center (ABC) of Global Frontier Project funded by the Ministry of Education, Science and Technology (ABC-2012-053880).

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Authors and Affiliations

  1. Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, Daejeon, 305-343, Korea

    Sun-A Choi, Jin-Suk Lee & Ji-Yeon Park

  2. Department of Chemical and Biological Engineering, Korea University, Seoul, 136-701, Korea

    Sun-A Choi & Seung Wook Kim

  3. Advanced Biomass R&D Center, KAIST, Daejeon, 305-701, Korea

    Joo-Young Jung & Ji-Won Yang

  4. Department of Chemical and Biomolecular Engineering, KAIST, Daejeon, 305-701, Korea

    Kyochan Kim, Jong-Hee Kwon & Ji-Won Yang

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  1. Sun-A Choi
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  2. Joo-Young Jung
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  5. Jin-Suk Lee
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Corresponding authors

Correspondence to Ji-Yeon Park or Ji-Won Yang.

Additional information

S.-A. Choi, J.-Y. Jung contributed equally to this work.

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Choi, SA., Jung, JY., Kim, K. et al. Effects of molten-salt/ionic-liquid mixture on extraction of docosahexaenoic acid (DHA)-rich lipids from Aurantiochytrium sp. KRS101. Bioprocess Biosyst Eng 37, 2199–2204 (2014). https://doi.org/10.1007/s00449-014-1197-2

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  • DOI: https://doi.org/10.1007/s00449-014-1197-2

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