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Effect of temperature and hydraulic retention time on volatile fatty acid production based on bacterial community structure in anaerobic acidogenesis using swine wastewater

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Abstract

To investigate the effect of hydraulic retention time (HRT) and temperature (T) on bacterial community structure and volatile fatty acids (VFAs) production of an acidogenic process, and VFA production and changes in the bacterial community in three identical automated anaerobic continuously-stirred tank reactors were analyzed using response surface analysis (RSA) and nonmetric multidimensional scaling (NMDS). For RSA, 11 trials were conducted to find the combination of T and HRT under which VFA production was greatest; VFA production was affected more by HRT than by T. To identify the bacterial community structure in each trial, DNA from each experimental point of the RSA was analyzed using denaturating gradient gel electrophoresis (DGGE), and eight bacteria species were detected. NMDS was conducted on band intensities obtained using DGGE, and bacterial community structure was affected more by T than by HRT. Taken together, these results suggest that VFA production during acidogenesis was more dependent on the physicochemical properties of acidogens, such as their specific growth rate or contact time with of substrates, than on changes in the microbial community.

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Abbreviations

ANOVA:

Analysis of variance

COD:

Chemical oxygen demand

CSTR:

Continuously stirred tank reactors

DDW:

Deionized and distilled water

DGGE:

Denaturating gradient gel electrophoresis

HRT:

Hydraulic retention time

NMDS:

Nonmetric multidimensional scaling

OD:

Optical density

RSA:

Response surface analysis

RSM:

Response surface methodology

sCOD:

Soluble chemical oxygen demand

T:

Temperature

TS:

Total solids

TSS:

Total suspended solids

TVFA:

Total volatile fatty acids

UPGMA:

Unweighed pair group method with arithmetic

VFA:

Volatile fatty acid

VS:

Volatile solids

VSS:

Volatile suspended solids

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Acknowledgments

This work was supported by the Advanced Biomass R&D Center (ABC) of Global Frontier Project funded by the Ministry of Education, Science and Technology (ABC-2010-0029728), and was also the New & Renewable Energy of the Korea Institute of Energy Technology Evaluation and Planning(KETEP) grant funded by the Korea government Ministry of Knowledge Economy (Grant no. 20103020090050).

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Authors and Affiliations

  1. School of Environmental Science and Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk, 790-784, Republic of Korea

    Seokhwan Hwang

  2. Infrastructure and Environment Division, School of Engineering, University of Glasgow, Glasgow, G12 8LT, UK

    Seung Gu Shin

  3. Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701, Republic of Korea

    Woong Kim

  4. POSCO, Gangnam-gu, Seoul, Republic of Korea

    Juntaek Lim

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  1. Woong Kim
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  2. Seung Gu Shin
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  3. Juntaek Lim
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  4. Seokhwan Hwang
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Correspondence to Seokhwan Hwang.

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Kim, W., Shin, S.G., Lim, J. et al. Effect of temperature and hydraulic retention time on volatile fatty acid production based on bacterial community structure in anaerobic acidogenesis using swine wastewater. Bioprocess Biosyst Eng 36, 791–798 (2013). https://doi.org/10.1007/s00449-013-0905-7

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  • DOI: https://doi.org/10.1007/s00449-013-0905-7

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