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Impact of oxygen level in gaseous phase on gene transcription and ganoderic acid biosynthesis in liquid static cultures of Ganoderma lucidum

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Abstract

Liquid static cultivation of Ganoderma lucidum was previously found to be very efficient for improving the production of its valuable antitumor compound ganoderic acid (GA) (Fang and Zhong in Biotechnol Prog 18:51–54, 2002). In this work, effects of oxygen concentration within the range of 21–100% (v/v) in the gaseous phase on the mycelia growth, GA production, and gene transcription of key enzymes for GA biosynthesis in liquid static cultures of G. lucidum were investigated. A high cell density of 29.8 ± 1.7 g/l DW and total GA production of 1427.2 ± 74.2 mg/l were obtained under an optimal gaseous O2 level of 80%. The expression of 3-hydroxy-3-methyl-glutaryl-CoA reductase, squalene synthase and lanosterol synthase genes of GA biosynthetic pathway as detected by quantitative real-time PCR was also affected by the gaseous oxygen concentration in the liquid static culture. H2O2 was generated as reactive oxygen species in response to high oxygen concentrations in the gas phase, and it seemed to be involved in the regulation of GA biosynthesis. The information obtained in this study provided an insight into the role of gaseous O2 in the GA production and it will be helpful for further enhancing its productivity.

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Acknowledgments

Financial support from the National Natural Science Foundation of China (NSFC project No. 20776084, No. 30821005), the National High Technology R&D Program (863 project No. 2007AA021506) of the Ministry of Science & Technology of China, and the Shanghai Leading Academic Discipline Project (project Nos. B203 and B505) is gratefully acknowledged.

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, China

    Wen-Xian Zhang & Jian-Jiang Zhong

  2. Engineering Research Center of Industrial Microbiology, Ministry of Education, College of Life Sciences, Fujian Normal University, 350108, Fuzhou, China

    Wen-Xian Zhang

  3. Key Laboratory of Fermentation Engineering (Ministry of Education) and Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, 430068, Wuhan, China

    Ya-Jie Tang

  4. Key Laboratory of Microbial Metabolism, Ministry of Education, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, 200240, Shanghai, China

    Jian-Jiang Zhong

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  1. Wen-Xian Zhang
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Correspondence to Jian-Jiang Zhong.

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Zhang, WX., Tang, YJ. & Zhong, JJ. Impact of oxygen level in gaseous phase on gene transcription and ganoderic acid biosynthesis in liquid static cultures of Ganoderma lucidum . Bioprocess Biosyst Eng 33, 683–690 (2010). https://doi.org/10.1007/s00449-009-0379-9

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  • DOI: https://doi.org/10.1007/s00449-009-0379-9

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