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Expression and effects of epidermal growth factor on human periodontal ligament cells

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Abstract

Repair of damaged periodontal ligament (PDL) tissue is an essential challenge in tooth preservation. Various researchers have attempted to develop efficient therapies for healing and regenerating PDL tissue based on tissue engineering methods focused on targeting signaling molecules in PDL stem cells and other mesenchymal stem cells. In this context, we investigated the expression of epidermal growth factor (EGF) in normal and surgically wounded PDL tissues and its effect on chemotaxis and expression of osteoinductive and angiogenic factors in human PDL cells (HPDLCs). EGF as well as EGF receptor (EGFR) expression was observed in HPDLCs and entire PDL tissue. In a PDL tissue-injured model of rat, EGF and IL-1β were found to be upregulated in a perilesional pattern. Interleukin-1β induced EGF expression in HPDLCs but not EGFR. It also increased transforming growth factor-α (TGF-α) and heparin-binding EGF-like growth factor (HB-EGF) expression. Transwell assays demonstrated the chemotactic activity of EGF on HPDLCs. In addition, EGF treatment significantly induced secretion of bone morphogenetic protein 2 and vascular endothelial growth factor, and gene expression of interleukin-8 (IL-8), and early growth response-1 and -2 (EGR-1/2). Human umbilical vein endothelial cells developed well-formed tube networks when cultured with the supernatant of EGF-treated HPDLCs. These results indicated that EGF upregulated under inflammatory conditions plays roles in the repair of wounded PDL tissue, suggesting its function as a prospective agent to allow the healing and regeneration of this tissue.

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Acknowledgments

This study was supported by a grant from the Ministry of Education, Culture, Sports, Science and Technology, Japan (grant number: 23689077, 24390426, 24659848, 24792028, 25293388, and 25670811). We thank Drs. Monnouchi, Hasegawa, Yuda and Yoshida for their great support in the preparation of this work.

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Authors and Affiliations

  1. Department of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University, Fukuoka, Japan

    Yoko Teramatsu, Hideki Sugii, Atsushi Tomokiyo, Sayuri Hamano, Asuka Yuda, Naohide Yamamoto, Katsuaki Koori & Akifumi Akamine

  2. Department of Endodontology, Kyushu University Hospital, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan

    Hidefumi Maeda, Naohisa Wada & Akifumi Akamine

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  1. Yoko Teramatsu
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  2. Hidefumi Maeda
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  6. Naohisa Wada
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  7. Asuka Yuda
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  9. Katsuaki Koori
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  10. Akifumi Akamine
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Correspondence to Hidefumi Maeda.

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Teramatsu, Y., Maeda, H., Sugii, H. et al. Expression and effects of epidermal growth factor on human periodontal ligament cells. Cell Tissue Res 357, 633–643 (2014). https://doi.org/10.1007/s00441-014-1877-x

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  • DOI: https://doi.org/10.1007/s00441-014-1877-x

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