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QTLs for cell wall-bound phenolics in relation to the photosynthetic apparatus activity and leaf water status under drought stress at different growth stages of triticale

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Abstract

The present study aimed at identifying the regions of triticale genome responsible for cell wall saturation with phenolic compounds under drought stress during vegetative and generative growth. Moreover, the loci determining the activity of the photosynthetic apparatus, leaf water content (LWC) and osmotic potential (Ψ o) were identified, as leaf hydration and functioning of the photosynthetic apparatus under drought are associated with the content of cell wall-bound phenolics (CWPh). Compared with LWC and Ψ o, CWPh fluctuations were more strongly associated with changes in chlorophyll fluorescence. At the vegetative stage, CWPh fluctuations were due to the activity of three loci, of which only QCWPh.4B was also related to changes in F v/F m and ABS/CSm. In the other QTLs (QCWPh.6R.2 and QCWPh.6R.3), the genes of these loci determined also the changes in majority of chlorophyll fluorescence parameters. At the generative stage, the changes in CWPh in loci QCWPh.4B, QCWPh.3R and QCWPh.6R.1 corresponded to those in DIo/CSm. The locus QCWPh.6R.3, active at V stage, controlled majority of chlorophyll fluorescence parameters. This is the first study on mapping quantitative traits in triticale plants exposed to drought at different stages of development, and the first to present the loci for cell wall-bound phenolics.

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Authors and Affiliations

  1. Polish Academy of Sciences, The Franciszek Górski Institute of Plant Physiology, Niezapominajek 21, 30-239, Kraków, Poland

    Tomasz Hura, Agnieszka Ostrowska & Kinga Dziurka

  2. Department of Biochemistry and Biotechnology, Rzeszów University of Technology, Albigowa 472, 37-122, Albigowa, Poland

    Mirosław Tyrka

  3. Department of Plant Physiology, Faculty of Agriculture and Economics, Agricultural University, Podłużna 3, 30-239, Kraków, Poland

    Katarzyna Hura

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  1. Tomasz Hura
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  2. Mirosław Tyrka
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  4. Agnieszka Ostrowska
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Correspondence to Tomasz Hura.

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Funding

The study was supported by the National Centre for Research and Development (Poland), Project GENMARK (PBS1/A8/1/2012; PBS1 177 150).

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All authors have no conflicts of interest to declare.

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This article does not describe any studies with human participants or animals performed by any of the authors.

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Communicated by S. Hohmann.

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438_2016_1276_MOESM1_ESM.xlsx

Appendix S1. QTL_source_sequences: Sequences of DArTseq markers and DArT clones for 5 QTLs influencing cell wall phenolic compounds content in triticale mapping population of Hewo x Magnat. (XLSX 20 kb)

438_2016_1276_MOESM2_ESM.xlsx

Appendix S2. URGI-Contigs: Contigs identified after BLASTing of DArTseq markers (from regions of QCWPh) vs. wheat genome deposited at URGI database. (XLSX 14 kb)

438_2016_1276_MOESM3_ESM.xlsx

Appendix S3. NCBI-BLAST: Complete list of genes from NCBI database matching contig sequences corresponding to regions affecting CWPh. (XLSX 21 kb)

438_2016_1276_MOESM4_ESM.jpg

Figure S1. A two-dimensional plot based on a principal coordinate analysis of phenotypic variation in Hewo x Magnat mapping population at the vegetative stage (V). (JPEG 382 kb)

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Figure S2. A two-dimensional plot based on a principal coordinate analysis of phenotypic variation in Hewo x Magnat mapping population at the generative stage (G). (JPEG 411 kb)

438_2016_1276_MOESM6_ESM.docx

Table S1. Summary statistics for all studied traits of the two parental lines and their DH lines at the vegetative (V) and generative (G) developmental stages of triticale growth. (DOCX 15 kb)

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Table S2. Coefficients and eigenvalues for the first 6 principal components based on Pearson correlation matrix between traits at the vegetative stage (V). (DOCX 17 kb)

438_2016_1276_MOESM8_ESM.docx

Table S3. Coefficients and eigenvalues for the first 6 principal components based on Pearson correlation matrix between traits at the generative stage (G). (DOCX 14 kb)

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Hura, T., Tyrka, M., Hura, K. et al. QTLs for cell wall-bound phenolics in relation to the photosynthetic apparatus activity and leaf water status under drought stress at different growth stages of triticale. Mol Genet Genomics 292, 415–433 (2017). https://doi.org/10.1007/s00438-016-1276-y

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