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Molecular characterization of Sp110 gene in pigs

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Abstract

Speckled 110 kDa (Sp110) plays an important role in infectious diseases, as revealed by studies in humans. However, little is known regarding porcine Sp110. To elucidate its potential role in porcine resistance to viral diseases, here, the complete coding sequence of porcine Sp110 gene and its 26 alternatively spliced isoforms were isolated using reverse transcription (RT)-polymerase chain reaction (PCR), and another seven splicing patterns were obtained using a minigene construct. Subcellular distribution of 11 representative isoforms was characterized in PK-15 cells transiently transfected with their respective GFP fusion constructs, and only isoforms (R and V) bearing all functional domains were localized in nucleus, indicating all the other isoforms lose normal functions of Sp110 owing to alternative splicing. Real-time quantitative PCR and competitive RT-PCR showed that both isoforms R and V had similar tissue expression profile, half-life and response to poly(I:C), a synthetic analog of viral double-stranded RNA, while the longer one (isoform R) was transcribed at a higher level. The results indicated that porcine Sp110 has a role in viral infection and that isoform R is the dominant active form. Overall the data provide potential resource for molecular breeding of pig resistant to diseases and contributes to breeding pigs resistant to viral infection.

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Funding

This study was funded by the National Natural Science Foundation of China (31072007) and Northeast Agricultural University Innovation Foundation for Postgraduate (yjscx14011).

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    Authors and Affiliations

    1. College of Animal Science and Technology, Northeast Agricultural University, Harbin, China

      Li-zu Li, Qiu-shi Wang, Li-xin Han, Jin-kui Wang, Si-yu Shao & Xiu-qin Yang

    2. Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin, China

      Liang Wang & Di Liu

    Authors
    1. Li-zu Li
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    2. Qiu-shi Wang
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    4. Jin-kui Wang
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    7. Di Liu
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    Correspondence to Di Liu or Xiu-qin Yang.

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    Conflict of interest

    Authors Li-zu Li, Qiu-shi Wang, Li-xin Han, Jin-kui Wang, Si-yu Shao, Liang Wang, Di Liu and Xiu-qin Yang declare no conflict of interest.

    Ethical approval

    All animal procedures were conducted according to guidelines on the Care and Use of Laboratory Animals of Heilongjiang Province, China.

    Additional information

    Communicated by M. Fredholm.

    Li-zu Li and Qiu-shi Wang have contributed equally to the work.

    Nucleotide sequence data reported are available in the GenBank database under the accession numbers KF898045, JX280458, and KC811303-811327.

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    Li, Lz., Wang, Qs., Han, Lx. et al. Molecular characterization of Sp110 gene in pigs. Mol Genet Genomics 291, 1431–1442 (2016). https://doi.org/10.1007/s00438-016-1189-9

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    • DOI: https://doi.org/10.1007/s00438-016-1189-9

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