Abstract
Long non-coding RNAs (lncRNAs) are an important class of pervasive genes involved in a variety of biological functions. It can serve as key co-activators of proteins involved in transcriptional regulation. Studies have found that white and brown adipocytes both originate from the mesoderm. However, it remains unclear whether lncRNAs function during adipogenesis or in energy metabolism in brown adipose tissue (BAT) and white adipose tissue (WAT). In this study, we used lncRNA microarray technology to evaluate differences in the lncRNA expression profiles of WAT and BAT. We observed 735 up-regulated and 877 down-regulated lncRNAs (fold change >4.0). To reveal the potential functions of these lncRNAs, we applied GO and pathway analyses to study the differentially expressed lncRNAs. We found that AK142386 and AK133540 may affect adipogenesis and metabolism. Our data indicate that AK142386 and AK133540 may be involved in BAT and WAT development through their target genes Hoxa3 and Acad10. Together, we have identified numerous lncRNAs and these lncRNAs can potentially serve as a required component for proper adipogenesis.
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Acknowledgments
This study was supported by grants from the National Key Basic Research Program of China (2013CB530604), the Key project of the National Natural Science Foundation of China (81330067), the National Natural Science Foundation of China (81270928, 81200642 and 81300683) and the Program for Innovative Research Teams of Jiangsu Province (LJ201108).
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Communicated by S. Hohmann.
J. Chen and X. Cui contributed equally to this work.
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438_2014_954_MOESM1_ESM.tif
Supplementary material 1 (TIFF 12780 kb) (A) AK142386 is located downstream of the intron of an Hoxa3 gene. (B) AK133540 is located downstream of an intrn of the Acad10 gene. (A and B) H3K4me1, H3K4me3, H3K27me3 and H3K27a tracks summarize the covalent histone modifications mapped by ChIP-Seq
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Chen, J., Cui, X., Shi, C. et al. Differential lncRNA expression profiles in brown and white adipose tissues. Mol Genet Genomics 290, 699–707 (2015). https://doi.org/10.1007/s00438-014-0954-x
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DOI: https://doi.org/10.1007/s00438-014-0954-x