Abstract
In previous studies we and others have found that activation of ryanodine receptors (RyRs) facilitate expression of long-term potentiation (LTP) of reactivity to afferent stimulation in hippocampal slices, with a more pronounced action in the ventral hippocampus. We have also been able to link the involvement of synaptopodin (SP), an actin-binding protein, with neuronal plasticity via its interaction with RyRs. To test this link more directly, we have now compared the ability of ryanodine to convert short-term to LTP in hippocampal slices taken from normal and SP-knockout (SPKO) mice. Indeed, SPKO hippocampus expresses lower concentrations of RyRs and in slices of these mice ryanodine is unable to facilitate conversion of short-term to LTP. These observations link functionally SP with calcium stores.
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Acknowledgments
Supported by a grant from German/Israel Foundation #1088-21/2010 to MS. We wish to thank Ms. E. Biton for the immunostaining, and Ms. S. Dayan for participating in the immunohistochemical analysis.
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The authors declare no conflict of interest.
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Grigoryan, G., Segal, M. Ryanodine-mediated conversion of STP to LTP is lacking in synaptopodin-deficient mice. Brain Struct Funct 221, 2393–2397 (2016). https://doi.org/10.1007/s00429-015-1026-7
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DOI: https://doi.org/10.1007/s00429-015-1026-7