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Molecular composition of extracellular matrix in the vestibular nuclei of the rat

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Abstract

Previous studies have demonstrated that the molecular and structural composition of the extracellular matrix (ECM) shows regional differences in the central nervous system. By using histochemical and immunohistochemical methods, we provide here a detailed map of the distribution of ECM molecules in the vestibular nuclear complex (VNC) of the rat. We have observed common characteristics of the ECM staining pattern in the VNC and a number of differences among the individual vestibular nuclei and their subdivisions. The perineuronal net (PNN), which is the pericellular condensation of ECM, showed the most intense staining for hyaluronan, aggrecan, brevican and tenascin-R in the superior, lateral and medial vestibular nuclei, whereas the HAPLN1 link protein and the neurocan exhibited moderate staining intensity. The rostral part of the descending vestibular nucleus (DVN) presented a similar staining pattern in the PNN, with the exception of brevican, which was negative. The caudal part of the DVN had the weakest staining for all ECM molecules in the PNN. Throughout the VNC, versican staining in the PNN, when present, was distinctive due to its punctuate appearance. The neuropil also exhibited heterogeneity among the individual vestibular nuclei in ECM staining pattern and intensity. We find that the heterogeneous distribution of ECM molecules is associated in many cases with the variable cytoarchitecture and hodological organization of the vestibular nuclei, and propose that differences in the ECM composition may be related to specific neuronal functions associated with gaze and posture control and vestibular compensation.

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Abbreviations

BSA:

Bovine serum albumin

CNS:

Central nervous system

CSPG:

Chondroitin sulfate proteoglycan

DVN:

Descending vestibular nucleus

ECM:

Extracellular matrix

GAG:

Glycosaminoglycan

GP:

Glycoprotein

HA:

Hyaluronan

HAPLN1:

Hyaluronan and proteoglycan link protein 1

LVN:

Lateral vestibular nucleus

NGS:

Normal goat serum

NHS:

Normal horse serum

NRS:

Normal rabbit serum

MVN:

Medial vestibular nucleus

PG:

Proteoglycan

PNN:

Perineuronal net

SVN:

Superior vestibular nucleus

TN-R:

Tenascin-R

VNC:

Vestibular nuclear complex

WFA:

Wisteria floribunda agglutinin

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Acknowledgments

The authors thank Ms. Timea Horvath for skillful technical assistance. Grant sponsor: MTA-TKI 255, DE OEC Bridging Fund and TÁMOP-4.2.2/B-10/1-2010-0024.

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Department of Anatomy, Histology and Embryology, Faculty of Medicine, Medical and Health Science Center, University of Debrecen, Nagyerdei krt. 98, Debrecen, 4032, Hungary

    Éva Rácz, Botond Gaál, Szilvia Kecskes & Clara Matesz

  2. Division of Oral Anatomy, Faculty of Dentistry, Medical and Health Science Center, University of Debrecen, Nagyerdei krt. 98, Debrecen, 4032, Hungary

    Botond Gaál & Clara Matesz

  3. MTA-DE Neuroscience Research Group, Nagyerdei krt. 98, Debrecen, 4032, Hungary

    Clara Matesz

Authors
  1. Éva Rácz
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  2. Botond Gaál
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  3. Szilvia Kecskes
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  4. Clara Matesz
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Corresponding author

Correspondence to Clara Matesz.

Additional information

É. Rácz and B. Gaál contributed equally to this work.

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Rácz, É., Gaál, B., Kecskes, S. et al. Molecular composition of extracellular matrix in the vestibular nuclei of the rat. Brain Struct Funct 219, 1385–1403 (2014). https://doi.org/10.1007/s00429-013-0575-x

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  • DOI: https://doi.org/10.1007/s00429-013-0575-x

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