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Dysregulation of the Rb pathway in recurrent pleomorphic adenoma of the salivary glands

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Abstract

Pleomorphic adenoma (PA) is the most common salivary gland neoplasm, and while mostly benign, recurrences (RPA) and malignant transformation to carcinoma ex-PA (CXPA) do occur. Cell cycle proteins important in its tumorigenesis have been studied as markers for PA with a high risk of RPA or CXPA. The aim of the present study was to investigate cell cycle markers p-16, cyclin D1, CDK4, E2F, and retinoblastoma (Rb) in this context. Expression of p16, cyclin D1, E2F, CDK4, and Rb was studied by immunohistochemistry in 24 cases of PA, 21 of RPA, and 2 of CXPA. The presence of HPV was assessed by in situ hybridization. Immunostaining for p16 and cyclin D1 was negative or weakly positive in most cases of PA while strongly positive in the majority of RPA and both CXPA cases. Staining for Rb and CDK4 was either negative or weakly positive in PA, RPA, and CXPA. Expression of E2F was stronger in RPA and CXPA than in PA. Nuclear reactivity for HPV was not observed in any case. In conclusion, the strong staining for p16, cyclinD1, and E2F in RPA and CXPA, while weak or negative in PA, suggests that these proteins might be involved in recurrence and malignant transformation of PA.

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Acknowledgments

The authors wish to thank Jeruza Pinheiro da Silveira Bossonaro and Nadir Freitas for their excellent technical expertise and assistance. Dr. Amy Brown is acknowledged for her help with the final English revision. This study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (number 12/00757-1).

Conflict of interest

The authors hereby declare no conflicts of interest.

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Authors and Affiliations

  1. Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Rua José Rocha Junqueira13 Ponte Preta, Campinas, SP, 13045-755, Brazil

    Ana Amélia de Souza, Fabricio Passador-Santos, Ney Soares de Araujo, Vera Cavalcanti de Araújo & Andresa Borges Soares

  2. Department of Pathology, School of Medicine, State University of Campinas (UNICAMP), Campinas, SP, Brazil

    Albina Altemani

  3. Department of Statistics, São Leopoldo Mandic Institute and Research Center, Campinas, SP, Brazil

    Cecilia Pedroso Turssi

Authors
  1. Ana Amélia de Souza
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  2. Albina Altemani
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Correspondence to Andresa Borges Soares.

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de Souza, A.A., Altemani, A., Passador-Santos, F. et al. Dysregulation of the Rb pathway in recurrent pleomorphic adenoma of the salivary glands. Virchows Arch 467, 295–301 (2015). https://doi.org/10.1007/s00428-015-1804-x

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  • DOI: https://doi.org/10.1007/s00428-015-1804-x

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