Abstract
Clear cell renal cell carcinomas (ccRCC) have aberrant signalling pathways which affect vascular endothelial growth factor and are related to increased tumour angiogenesis. Little is known about other angiogenesis-associated genes in primary tumours and metastases. Quantitative PCR of 45 angiogenesis-associated gene transcripts was performed on formalin-fixed and paraffin-embedded tissues from primary ccRCC (n = 18) and their metastases (n = 17; in 8/17 cases the corresponding primary tumour could be analysed). In metastases, a significant increase was found in the expression of 15 pro-angiogenic (such as prostaglandin-endoperoxide synthase 1) and also anti-angiogenic (such as TIMP metallopeptidase inhibitor 2) factors. Comparison of a primary with its metastasis performed on eight cases showed that even without preceding anti-angiogenic therapy in metastases expression of angiogenic factors is increased. In ccRCC, the effects of anti-angiogenic factors are superimposed by pro-angiogenic factors. Increased expression of angiogenic factors in metastases might be related to development of resistance after anti-angiogenic therapy but might also be an inherent biological characteristic.
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Histomorphology (MA, HK), molecular analysis (ASK, MA, KH), data collection, analysis of data and manuscript preparation (MA, JS, MR, VG, TH, DJ, HK, KH).
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Abbas, M., Salem, J., Stucki-Koch, A. et al. Expression of angiogenic factors is increased in metastasised renal cell carcinomas. Virchows Arch 464, 197–202 (2014). https://doi.org/10.1007/s00428-013-1529-7
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DOI: https://doi.org/10.1007/s00428-013-1529-7