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Indolacetic and humic acids induce lateral root development through a concerted plasmalemma and tonoplast H+ pumps activation

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Abstract

Increasing evidences have indicated that humic substances can induce plant growth and productivity by functioning as an environmental source of auxinic activity. Here we comparatively evaluate the effects of indole-3-acetic acid (IAA) and humic acids (HA) isolated from two different soils (Inseptsol and Ultisol) and two different organic residues (vermicompost and sewage sludge) on root development and on activities of plasmalemma and tonoplast H+ pumps from maize roots. The data show that HA isolated from these different sources as well as low IAA concentrations (10−10 and 10−15 M) improve root growth through a markedly proliferation of lateral roots along with a differential activation not only of the plasmalemma but also of vacuolar H+-ATPases and H+-pyrophosphatase. Further, the vacuolar H+-ATPase had a peak of stimulation in a range from 10−8 to 10−10 M IAA, whereas the H+-pyrophosphatase was sensitive to a much broader range of IAA concentrations from 10−3 to 10−15 M. It is proposed a complementary view of the acid growth mechanism in which a concerted activation of the plasmalemma and tonoplast H+ pumps plays a key role in the root cell expansion process driven by environment-derived molecules endowed with auxinic activity, such as that of humic substances.

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Abbreviations

HA:

Humic acids

H+-PPase:

Membrane-bound pyrophosphatase

IAA:

Indole-3-acetic acid

P-ATPase:

Plasma membrane H+-adenosine triphosphatase

V-ATPase:

Vacuolar H+-adenosine triphosphatase

V-PPase:

Vacuolar H+-pyrophosphatase

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Acknowledgments

The authors would like to thank Prof. Anna L. Okorokova-Façanha (UENF, Rio de Janeiro, Brazil) for critical revision of the manuscript. We are also indebted to two anonymous referees for valuable comments and suggestions. This work was supported by the International Foundation for Science (IFS), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ).

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  1. Laboratório de Biologia Celular e Tecidual (LBCT), Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense (UENF), Campos dos Goytacazes, Rio de Janeiro, CEP 28013-600, Brazil

    Daniel Basílio Zandonadi & Arnoldo Rocha Façanha

  2. Laboratório de Solos, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, CEP 28013-600, Brazil

    Luciano Pasqualoto Canellas

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  1. Daniel Basílio Zandonadi
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  2. Luciano Pasqualoto Canellas
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Correspondence to Arnoldo Rocha Façanha.

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Zandonadi, D.B., Canellas, L.P. & Façanha, A.R. Indolacetic and humic acids induce lateral root development through a concerted plasmalemma and tonoplast H+ pumps activation. Planta 225, 1583–1595 (2007). https://doi.org/10.1007/s00425-006-0454-2

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