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Functional assessment of three Rem residues identified as critical for interactions with Ca2+ channel β subunits

  • Ion channels, receptors and transporters
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Pflügers Archiv - European Journal of Physiology Aims and scope Submit manuscript

Abstract

Members of the Rem, Rem2, Rad, Gem/Kir (RGK) family of small GTP-binding proteins inhibit high-voltage-activated (HVA) Ca2+ channels through interactions with both the principal α1 and the auxiliary β subunits of the channel complex. Three highly conserved residues of Rem (R200, L227, and H229) have been shown in vitro to be critical for interactions with β subunits. However, the functional significance of these residues is not known. To investigate the contributions of R200, L227, and H229 to β subunit-mediated RGK protein-dependent inhibition of HVA channels, we introduced alanine substitutions into all three positions of Venus fluorescent protein-tagged Rem (V-Rem AAA) and made three other V-Rem constructs with an alanine introduced at only one position (V-Rem R200A, V-Rem L227A, and V-Rem H229A). Confocal imaging and immunoblotting demonstrated that each Venus-Rem mutant construct had comparable expression levels to Venus-wild-type Rem when heterologously expressed in tsA201 cells. In electrophysiological experiments, V-Rem AAA failed to inhibit N-type Ca2+ currents in tsA201 cells coexpressing CaV2.2 α1B, β3, and α2δ-1 channel subunits. The V-Rem L227A single mutant also failed to reduce N-type currents conducted by coexpressed CaV2.2 channels, a finding consistent with the previous observation that a leucine at position 227 is critical for Rem-β interactions. Rem-dependent inhibition of CaV2.2 channels was impaired to a much lesser extent by the R200A substitution. In contrast to the earlier work demonstrating that Rem H229A was unable to interact with β3 subunits in vitro, V-Rem H229A produced nearly complete inhibition of CaV2.2-mediated currents.

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Acknowledgments

We thank Dr. U. Meza for comments on the manuscript, Drs. B.A. Adams. S.R. Ikeda, P.J. Kammermeier, H.L. Puhl, III, and W.A. Sather for sharing expression plasmids and Drs. K.G. Beam and D. Oskar for continued support. This work was supported by grants from the American Heart Association (13BGIA13630000) and the Boettcher Foundation to R.A.B.. D.B. received a stipend from 2T32AG000279-11 (to R.S. Schwartz, University of Colorado Denver-AMC Department of Medicine-Geriatrics Division). Confocal images were acquired in the University of Colorado Denver-AMC Advanced Light Microscopy Core (funded in part by NIH/NCRR Colorado CTSI Grant Number UL1 RR025780).

Compliance with Ethical Standards

Disclosure of potential conflicts of interest: None of the authors have a competing interest. Research involving Human Participants and/or Animals: N/A. Informed consent: N/A.

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Authors and Affiliations

  1. Department of Medicine-Cardiology Division, School of Medicine, University of Colorado Denver-Anschutz Medical Campus, 12700 East 19th Avenue, P15-8006, B-139, Aurora, CO, 80045, USA

    Donald Beqollari, Christin F. Romberg & Roger A. Bannister

  2. Department of Vegetative Physiology, University of Cologne, Robert-Koch-Str. 39, D-50931, Cologne, Germany

    Dilyana Filipova & Symeon Papadopoulos

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  1. Donald Beqollari
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  2. Christin F. Romberg
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  5. Roger A. Bannister
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Correspondence to Roger A. Bannister.

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Beqollari, D., Romberg, C.F., Filipova, D. et al. Functional assessment of three Rem residues identified as critical for interactions with Ca2+ channel β subunits. Pflugers Arch - Eur J Physiol 467, 2299–2306 (2015). https://doi.org/10.1007/s00424-015-1700-x

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