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High glucose concentrations stimulate human monocyte sodium/hydrogen exchanger activity and modulate atherosclerosis-related functions

  • Ion Channels, Transporters
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Abstract

In the present study, the effect of high (20 mM) glucose concentrations on human monocyte sodium/hydrogen exchanger (NHE1) activity, scavenger receptor CD36 expression, cell adhesion, and cell migration have been investigated. Incubation with high glucose concentrations caused an increase in NHE1 activity, as estimated by internal pH and sodium-uptake measurements. This effect was specific for glucose, since it was not observed when monocytes were incubated in the presence of 20 mM of galactose, fructose, or mannitol. In addition, the activation of sodium uptake was inhibited by ethylisopropyl amiloride (EIPA), phloretine and cytochalasine B, and calphostin C. High glucose concentrations also increased the expression of CD36 receptors on the surface of monocytes and positively influenced monocyte migration and adhesion to laminin. EIPA added together with glucose counteracted these effects. The data of the present study suggest that a high glucose concentration can influence atherosclerosis-related monocyte functions via NHE1 activation.

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Acknowledgements

The authors thank Ms. Melpomeni Christophoridou for excellent technical and secretarial assistance.

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Authors and Affiliations

  1. Department of Biological Chemistry, Medical School, Aristotle University of Thessaloniki, PO Box 17034, 54124 , Thessaloniki, Greece

    G. Koliakos

  2. Laboratory of Animal Physiology, Department of Biology, School of Science, Aristotle University of Thessaloniki, 54124 , Thessaloniki, Greece

    Z. Zolota & M. Kaloyianni

  3. Laboratory for the Study of Metabolic Diseases, B’ Medical Clinic, Medical School, Aristotle University of Thessaloniki, 54124 , Thessaloniki, Greece

    K. Paletas

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  1. G. Koliakos
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  2. Z. Zolota
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  3. K. Paletas
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  4. M. Kaloyianni
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Correspondence to G. Koliakos.

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Koliakos, G., Zolota, Z., Paletas, K. et al. High glucose concentrations stimulate human monocyte sodium/hydrogen exchanger activity and modulate atherosclerosis-related functions. Pflugers Arch - Eur J Physiol 449, 298–306 (2004). https://doi.org/10.1007/s00424-004-1340-z

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  • DOI: https://doi.org/10.1007/s00424-004-1340-z

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