Skip to main content
SpringerLink
Log in

Changes in von Willebrand factor and fibrinolysis following a post-exercise cool-down

  • Original Article
  • Published:
European Journal of Applied Physiology Aims and scope Submit manuscript

Abstract

The purpose of this study was to determine the effect of a post-exercise active cool-down on von Willebrand factor and fibrinolysis. Ten subjects performed two maximal oxygen uptake (V̇O2max) tests followed by a 10-min passive (PC) or an active (AC) cool-down. Blood samples were obtained pre-exercise, post-exercise, post-PC/AC, and 1 h post-exercise and analyzed for von Willebrand factor antigen (vWf:Ag), tissue plasminogen activator (tPA) antigen and activity and plasminogen activator inhibitor-1 (PAI-1) activity. Data were analyzed using repeated measures analysis of variance. No significant differences were found between V̇O2max tests for treadmill time, V̇O2max, respiratory exchange ratio, maximal heart rate, or maximal blood lactate concentration. vWf:Ag was significantly elevated (P <0.05) following PC [198.4 (18.3)% normal] versus AC [174.5 (15.6)% normal] and remained elevated 1-h post-exercise [179.4 (16.4)% normal for PC vs 158.6 (13.8)% normal for AC]. There were no differences between tests for tPA or PAI-1 activity, although tPA antigen was significantly elevated following PC versus AC (P <0.05). Following the cool-down, hematocrit was higher (P <0.05) for the PC test [48.90 (0.36)] compared with AC [47.43 (0.51)]. An AC reduces post-exercise vWf:Ag and tPA antigen without affecting tPA or PAI-1 activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Andrew M, Carter C, O’Brodovich H, Heigenhauser G (1986) Increases in factor VIII complex and fibrinolytic activity are dependent on exercise intensity. J Appl Physiol 60:1917–1922

    CAS  PubMed  Google Scholar 

  • Angleton P, Chandler WL, Schmer G (1989) Diurnal variation of tissue-type plasminogen activator and its rapid inhibitor (PAI-1). Circulation 79:101–106

    CAS  PubMed  Google Scholar 

  • Bartsch P, Haeberli A, Straub PW (1990) Blood coagulation after long distance running: antithrombin III prevents fibrin formation. Thromb Haemost 63:430–434

    CAS  PubMed  Google Scholar 

  • Beaumont W Van, Greenleaf JE, Juhos L (1972) Disproportional changes in hematocrit, plasma volume, and proteins during exercise and bed rest. J Appl Physiol 33:55–61

    PubMed  Google Scholar 

  • Burg PJ van den, Hospers JE, van Vliet M, Mosterd WL, Bouma BN, Huisveld IA (1997) Effect of endurance training and seasonal fluctuation on coagulation and fibrinolysis in young sedentary men. J Appl Physiol 82: 613–620

    PubMed  Google Scholar 

  • Chandler WL, Veith RC, Fellingham GW, Levy WC, Schwartz RS, Cerqueira MD, Kahn SE, Larson VG, Cain KC, Beard JC, et al (1992) Fibrinolytic response during exercise and epinephrine infusion in the same subjects. J Am Coll Cardiol 19:1412–1420

    CAS  PubMed  Google Scholar 

  • Ciampricotti R, el-Gamal M, Relik T, Taverne R, Panis J, de Swart J, van Gelder B, Relik-van Wely L (1990) Clinical characteristics and coronary angiographic findings of patients with unstable angina, acute myocardial infarction, and survivors of sudden ischemic death occurring during and after sport. Am Heart J 120:1267–1278

    CAS  PubMed  Google Scholar 

  • Cohen RJ, Epstein SE, Cohen LS, Dennis LH (1968) Alterations of fibrinolysis and blood coagulation induced by exercise, and the role of beta-adrenergic-receptor stimulation. Lancet 2:1264–1266

    CAS  PubMed  Google Scholar 

  • Davis GL, Abildgaard CF, Bernauer EM, Britton M (1976) Fibrinolytic and hemostatic changes during and after maximal exercise in males. J Appl Physiol 40:287–292

    CAS  PubMed  Google Scholar 

  • Dimsdale JE, Hartley LH, Guiney T, Ruskin JN, Greenblatt D (1984) Postexercise peril. Plasma catecholamines and exercise. JAMA 251:630–632

    Article  CAS  PubMed  Google Scholar 

  • Duncan JJ, Farr JE, Upton SJ, Hagan RD, Oglesby ME, Blair SN (1985) The effects of aerobic exercise on plasma catecholamines and blood pressure in patients with mild essential hypertension. JAMA 254:2609–2613

    Article  CAS  PubMed  Google Scholar 

  • Franklin BA, Whaley MH, Howley ET (2000) ACSM’s guidelines for exercise testing and prescription. Lippincott Williams and Wilkins, Baltimore, Md.

  • Fujii Y, Mammen EF, Farag A, Muz J, Salciccioli GG, Weingarden ST (1992) Thrombosis in spinal cord injury. Thromb Res 68:357–368

    CAS  PubMed  Google Scholar 

  • Giri S, Thompson PD, Kiernan FJ, Clive J, Fram DB, Mitchel JF, Hirst JA, McKay RG, Waters DD (1999) Clinical and angiographic characteristics of exertion-related acute myocardial infarction. JAMA 282:1731–1736

    Article  CAS  PubMed  Google Scholar 

  • Hansen JB, Wilsgard L, Olsen JO, Osterud B (1990) Formation and persistence of procoagulant and fibrinolytic activities in circulation after strenuous physical exercise. Thromb Haemost 64:385–389

    CAS  PubMed  Google Scholar 

  • Hegde SS, Goldfarb AH, Hegde S (2001) Clotting and fibrinolytic activity change during the 1 h after a submaximal run. Med Sci Sports Exerc 33:887–892

    CAS  PubMed  Google Scholar 

  • Hilberg T, Glaser D, Reckhart C, Prasa D, Sturzebecher J, Gabriel HH (2003) Blood coagulation and fibrinolysis after long-duration treadmill exercise controlled by individual anaerobic threshold. Eur J Appl Physiol 90:639–642

    Article  CAS  PubMed  Google Scholar 

  • Kanel R von, Dimsdale JE, Adler KA, Dillon E, Perez CJ, Mills PJ (2003) Effects of nonspecific β-adrenergic stimulation and blockade on blood coagulation in hypertension. J Appl Physiol 94:1455–1459

    PubMed  Google Scholar 

  • Laurell CB (1966) Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal Biochem 15:45–52

    CAS  PubMed  Google Scholar 

  • Lin X, El-Sayed MS, Waterhouse J, Reilly T (1999) Activation and disturbance of blood haemostasis following strenuous physical exercise. Int J Sports Med 20:149–153

    CAS  PubMed  Google Scholar 

  • Mehta J, Mehta P, Lawson D, Saldeen T (1987) Plasma tissue plasminogen activator inhibitor levels in coronary artery disease: correlation with age and serum triglyceride concentrations. J Am Coll Cardiol 9:263–268

    CAS  PubMed  Google Scholar 

  • Mittleman MA, Maclure M, Tofler GH, Sherwood JB, Goldberg RJ, Muller JE (1993) Triggering of acute myocardial infarction by heavy physical exertion. Protection against triggering by regular exertion. Determinants of Myocardial Infarction Onset Study Investigators. N Engl J Med 329:1677–1683

    Article  CAS  PubMed  Google Scholar 

  • Mustonen P, Lepantalo M, Lassila R (1998) Physical exertion induces thrombin formation and fibrin degradation in patients with peripheral atherosclerosis. Arterioscler Thromb Vasc Biol 18:244–249

    CAS  PubMed  Google Scholar 

  • Prowse CV, Cash JD (1984) Physiologic and pharmacologic enhancement of fibrinolysis. Semin Thromb Hemost 10:51–60

    CAS  PubMed  Google Scholar 

  • Rennie JA, Bennett B, Ogston D (1977) Effect of local exercise and vessel occlusion on fibrinolytic activity. J Clin Pathol 30:350–352

    CAS  PubMed  Google Scholar 

  • Saenko EL, Yakhyaev AV, Mikhailenko I, Strickland DK, Sarafanov AG (1999) Role of the low density lipoprotein-related protein receptor in mediation of factor VIII catabolism. J Biol Chem 274:37685–37692

    Article  CAS  PubMed  Google Scholar 

  • Scurr JH, Smith PD, Machin S (2001) Deep vein thrombosis in airline passengers—the incidence of deep vein thrombosis and the efficacy of elastic compression stockings. Cardiovasc Surg 9:159–161

    Article  CAS  PubMed  Google Scholar 

  • Smith DT, Hoetzer GL, Greiner JJ, Stauffer BL, DeSouza CA (2003) Effects of ageing and regular aerobic exercise on endothelial fibrinolytic capacity in humans. J Physiol (Lond) 546:289–298

    Google Scholar 

  • Sweeney JD, Hoernig LA (1992) Intraplatelet von Willebrand factor and ABO blood group. Thromb Res 68:393–398

    CAS  PubMed  Google Scholar 

  • Weiss C, Seitel G, Bartsch P (1998) Coagulation and fibrinolysis after moderate and very heavy exercise in healthy male subjects. Med Sci Sports Exerc 30:246–251

    CAS  PubMed  Google Scholar 

  • Winther K, Hillegass W, Tofler GH, Jimenez A, Brezinski DA, Schafer AI, Loscalzo J, Williams GH, Muller JE (1992) Effects on platelet aggregation and fibrinolytic activity during upright posture and exercise in healthy men. Am J Cardiol 70:1051–1055

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Human Energy Research Laboratory, Department of Kinesiology, Michigan State University, East Lansing, MI 48824, USA

    C. M. Paton, P. R. Nagelkirk, A. M. Coughlin, J. A. Cooper, J. M. Pivarnik & C. J. Womack

  2. Department of Medical Technology, Michigan State University, East Lansing, MI 48824, USA

    G. A. Davis

  3. College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA

    H. Hassouna

Authors
  1. C. M. Paton
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. R. Nagelkirk
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. M. Coughlin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. A. Cooper
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. A. Davis
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. H. Hassouna
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. J. M. Pivarnik
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. C. J. Womack
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to C. J. Womack.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Paton, C.M., Nagelkirk, P.R., Coughlin, A.M. et al. Changes in von Willebrand factor and fibrinolysis following a post-exercise cool-down. Eur J Appl Physiol 92, 328–333 (2004). https://doi.org/10.1007/s00421-004-1098-1

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00421-004-1098-1

Keywords

Search

Navigation