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Soluble form of LR11 is highly increased in the vitreous fluids of patients with idiopathic epiretinal membrane

  • Retinal Disorders
  • Published:
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Abstract

Purpose

LR11 (also called SorLA or SORL1) is a migration regulator of adherent cells with the immature proliferative phenotype. The present study investigated the clinical and pathological involvement of the soluble form of LR11 (sLR11) in the idiopathic epiretinal membrane (iERM).

Methods

The subjects were 51 patients with iERM (24 cellophane macular reflex (CMR) and 27 preretinal macular fibrosis (PMF)) and 45 patients with macular holes as age and sex-matched controls. Vitreous sLR11 and transforming growth factor (TGF)β2 levels were measured by ELISA.

Results

The sLR11 levels in the vitreous fluids of patients with iERM (20.2 ± 8.1 ng/mL) were significantly higher than those in controls (11.4 ± 4.7 ng/mL). Among the patients with iERM, the vitreous sLR11 levels were significantly higher in PMF (23.6 ± 8.2 ng/mL), than those in CMR (16.5 ± 5.9 ng/mL). Multivariate regression analysis of the studied factors showed that sLR11 was a unique factor independently contributing to the discrimination of the iERM patients against the control subjects (odds ratio [OR] 1.35 per 1-ng/mL increase, 95% CI 1.09–1.67; P = 0.004). ROC analysis showed that the sensitivity and the specificity of sLR11, but not of other studied factors, categorized into the rank of moderate accuracy. Finally, there was a positive correlation (R = 0.588; P = 0.003) between the vitreous levels of sLR11 and TGFβ2 using the available samples.

Conclusions

sLR11 levels in vitreous fluids were specifically increased in patients with iERM, suggesting the involvement in the pathology of proliferative and migrating cells for the development of iERM.

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Acknowledgements

Design and conduct study: R.H. and H.B. Collection: R.H., T.S. and T.M.; management, analysis, and interpretation of the data: R.H., M.J., N.H., M.T., M.H., Y.H. and H.B.; and preparation, review, or approval of the manuscript: R.H., M.J., T.S., N.H., M.T., M.H., Y.H., H.B. and T.M. We had no statistical consultation or assistance.

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Authors and Affiliations

  1. Department of Ophthalmology, Sakura Medical Center of Toho University, 564-1, Shimoshizu, Sakura, 285-8741, Japan

    Ryuya Hashimoto & Takatoshi Maeno

  2. Department of Clinical-Laboratory and Experimental-Research Medicine, Toho University Sakura Medical Center, 564-1 Shimoshizu, Sakura-Shi, Chiba prefecture, 285-8741, Japan

    Meizi Jiang, Nobuyuki Hiruta & Hideaki Bujo

  3. Department of Ophthalmology, Toho University Omori Medical Center, Tokyo, 143-8541, Japan

    Tomoaki Shiba & Yuichi Hori

  4. Cardiovascular Center, Toho University Sakura Medical Center, Sakura, 285-8741, Japan

    Mao Takahashi

  5. Department of Pathology, Saitama Medical Center, Saitama Medical University, Kawagoe, 350-8550, Japan

    Morihiro Higashi

Authors
  1. Ryuya Hashimoto
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  2. Meizi Jiang
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Corresponding author

Correspondence to Hideaki Bujo.

Ethics declarations

All procedures performed in studies involving human participants were in accordance with the institutional review board of Toho University Sakura Medical Center (No.S16036) and with the 1964 Helsinki Declaration. Informed consent was obtained from all individual participants included in the study.

Funding

This study was supported, in part, by Grants-in–aid for Scientific Research to Hideaki Bujo (24390231 and 15 K15198) and Meizi Jiang (24790907), and by a supported program for the Strategic Research Foundation at Private Universities (S1411015) from Japanese Ministry of Education, Culture, Sports, Science and Technology. This study was also supported by Japan Health and Labour Sciences Research Grant to Hideaki Bujo (H22-rinkensui-ippan-001), and by the Toho University Research Fund to Ryuya Hashimoto.

Conflict of interest

All authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speaker’s bureaus; membership, employment, consultancies, stock ownership).

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Hashimoto, R., Jiang, M., Shiba, T. et al. Soluble form of LR11 is highly increased in the vitreous fluids of patients with idiopathic epiretinal membrane. Graefes Arch Clin Exp Ophthalmol 255, 885–891 (2017). https://doi.org/10.1007/s00417-017-3585-1

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  • DOI: https://doi.org/10.1007/s00417-017-3585-1

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