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A biomimetic collagen/heparin multi-layered porous hydroxyapatite orbital implant for in vivo vascularization studies on the chicken chorioallantoic membrane

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Abstract

Background

The vascularization of an orbital implant is a key issue for reducing complications, such as exposure and infection.

Methods

Here, we developed a facile layer-by-layer assembly approach to modify porous hydroxyapatite (pHA) orbital implants with five collagen (COL)/heparin (HEP) multilayers.

Results

SEM characterization showed that the average pore size of the pHA/(COL/HEP)5 scaffold was 316.8 ± 77.1 μm. After being coated with five COL/HEP multilayers, the mechanical strength was improved compared with that of the pHA scaffolds. The in vitro assay displayed that the pHA scaffolds covered with COL/HEP multilayers resulted in a larger number of human umbilical vein endothelial cells after being cultured for 14 days. The macroscopic evaluation and semi-quantitative vascular density analysis of the chicken chorioallantoic membrane assay showed that the pHA/(COL/HEP)5 scaffolds resulted in more intense angiogenesis than the pHA scaffolds.

Conclusions

These studies demonstrate that the biomembrane-mimicking coating of COL/HEP multilayers is a simple and effective strategy to endow combined biological performances of pHA orbital implants and to potentially reduce implant-related complications.

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Acknowledgments

This work was financially supported by the National “Twelfth Five-Year” Plan for Science & Technology Support of China (2012BAI08B01), Zhejiang Provincial Program for Cultivation of High-Level Innovative Health Talents, The Specialized Key Science & Technology Foundation of Zhejiang Provincial S & T Department (No.2012C13023-2), Zhejiang Key Laboratory fund of China (2011E10006) and Zhejiang Key Innovation Team Project of China (No.2009R50039). We thank Dr. Shaoze Wang (Institute of Translational Medicine, Zhejiang University) for assisting in digital segmentation.

Conflict of Interest

All authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest (such as honoraria, educational grants, participation in speakers’ bureaus, membership, employment, consultancies, stock ownership, or other equity interest, expert testimony, or patent-licensing arrangements), or non-financial interest (such as personal or professional relationships, affiliations, knowledge, or beliefs) in the subject matter or materials discussed in this manuscript.

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Authors and Affiliations

  1. Department of Ophthalmology, the Second Affiliated Hospital of Zhejiang University, College of Medicine, Hangzhou, 310009, China

    Kai Jin, Xin Ye & Juan Ye

  2. MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China

    Bo Li & Changyou Gao

  3. Key Laboratory of Molecular Animal Nutrition of the Ministry of Education and Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China

    Sha Li & Caiqiao Zhang

Authors
  1. Kai Jin
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  2. Xin Ye
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  3. Sha Li
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  4. Bo Li
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  6. Changyou Gao
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  7. Juan Ye
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Correspondence to Changyou Gao or Juan Ye.

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Jin, K., Ye, X., Li, S. et al. A biomimetic collagen/heparin multi-layered porous hydroxyapatite orbital implant for in vivo vascularization studies on the chicken chorioallantoic membrane. Graefes Arch Clin Exp Ophthalmol 254, 83–89 (2016). https://doi.org/10.1007/s00417-015-3144-6

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  • DOI: https://doi.org/10.1007/s00417-015-3144-6

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